PTFE適配器錐形付φ30試管用|柴田科技有限公司-環境檢測設備、科學儀器的制造銷售

產品詳細

科學儀器

PTFE適配器錐形付φ30試管用

商品代碼其他情報(式樣)

這個產品比較表中追加
商品代碼 054310 – 4420
型式
價格(不含稅) 12萬日元。

上海金畔生物科技有限公司

-27457%2F%3Fc%3D34& ” -27457%2F%3Fc%3D34&

日本WAKO 和光純藥 193-16571 Phos-tag? 預制膠50μmol/l,12.5%,17孔

Nard
——————

301-93531

Phos-tag? Biotin BTL-104

Phos-tag? 生物素

10 mg 8,570.00
Wako
——————
和光純藥

193-16571

SuperSep? Phos-tag? (50 μmol/L), 12.5%,17 well

Phos-tag? 預制膠50μmol/l,12.5%,17孔

5塊 3,370.00
Wako
——————
和光純藥

193-16691

SuperSep? Phos-tag? (50 μmol/L), 15%,13 well

Phos-tag? 預制膠50μmol/l,15%,13孔

5塊 3,370.00
Manac
——————

302-93561

Phos-tag? Agarose

Phos-tag? 瓊脂糖

0.5 ml 2,450.00
Nard
——————

387-07321-8P

Phos-tag? Tip

Phos-tag? 瓊脂糖吸管

8 pcs 2,470.00
Nard
——————

304-93521

Phos-tag? Acrylamide AAL-107

Phos-tag? 丙烯酰胺

10 mg 7,340.00
Wako
——————
和光純藥

197-16851

SuperSep? Phos-tag? (50 μmol/L), 17.5%,13 well

Phos-tag? 預制膠50μmol/l,17.5%,13孔

5塊 3,370.00
Nard
——————

304-93526

Phos-tag? Acrylamide AAL-107 5mM Aqueous Solution

Phos-tag? 丙烯酰胺5mM水溶液

0.3 ml 1,840.00
Nard
——————

305-93551

Phos-tag? Mass Analytical Kit

Phos-tag? 質譜分析試劑盒

1 set 12,240.00
Nard
——————

308-93541

Phos-tag? Biotin BTL-105

Phos-tag? 生物素BTL-105

10 mg 咨詢
Manac
——————

308-93563

Phos-tag? Agarose

Phos-tag? 瓊脂糖

3 ml 12,000.00
Nard
——————

308-97201

Phos-tag? Biotin BTL-111 1mM Aqueous Solution

Phos-tag? 生物素1mM水溶液

0.1 ml 2,450.00
Wako
——————
和光純藥

190-16721

SuperSep? Phos-tag? (50 μmol/L), 10%,17 well

Phos-tag? 預制膠50μmol/l,10%,17孔

5塊 3,370.00
Wako
——————
和光純藥

194-16861

SuperSep? Phos-tag? (50 μmol/L), 17.5%,17 well

Phos-tag? 預制膠50μmol/l,17.5%,17孔

5塊 3,370.00
Wako
——————
和光純藥

198-17981

SuperSep? Phos-tag? (50μmol/l), 7.5%, 17well, 83×100×3.9mm

Phos-tag? 預制膠50μmol/l,7.5%,17孔,BioRad型

for Electrophoresis 5 EA 3,370.00
Wako
——————
和光純藥

195-17991

SuperSep? Phos-tag? (50μmol/l), 12.5%, 17well, 83×100×3.9mm

Phos-tag? 預制膠50μmol/l,12.5%,17孔,BioRad型

for Electrophoresis 5 EA 3,370.00
Wako
——————
和光純藥

192-18001

SuperSep? Phos-tag? (50μmol/l), 7.5%, 17well, 100×100×6.6mm

Phos-tag? 預制膠50μmol/l,7.5%,17孔,Life Technologies型

for Electrophoresis 5 EA 3,370.00
Wako
——————
和光純藥

199-18011

SuperSep? Phos-tag? (50μmol/l), 12.5%, 17well, 100×100×6.6mm

Phos-tag? 預制膠50μmol/l,12.5%,17孔,Life Technologies型

for Electrophoresis 5 EA 3,370.00
Wako
——————
和光純藥

195-16391

SuperSep? Phos-tag? (50 μmol/L), 12.5%,13 well

Phos-tag? 預制膠50μmol/l,12.5%,13孔

5塊 3,370.00
Wako
——————
和光純藥

192-17381

SuperSep? Phos-tag? (50μmol/l), 7.5%, 17well

Phos-tag? 預制膠50μmol/l,7.5%,17孔

5塊 3,370.00

上海金畔生物科技有限公司提供Lumiprobe產品目錄報價

上海金畔生物科技有限公司提供Lumiprobe產品目錄報價

Catalog?no. Product?name Price?/?USD RMB TIME
615B0 1-Ethynyl?pyrene,?100?mg 65.00 780 in?stock
555B0 3-Ethynyl?perylene,?50?mg 65.00 780 in?stock
655B0 3-Ethynyl?perylene,?100?mg 110.00 1320 in?stock
21360 Alkyne?Amidite,?hydroxyprolinol,?250?mg 240.00 2880 21?days
41360 Alkyne?Amidite,?hydroxyprolinol,?1?g 560.00 6720 21?days
22860 Alkyne?CPG?modifier?500,?250?mg 120.00 1440 21?days
42860 Alkyne?CPG?modifier?500,?1?g 380.00 4560 21?days
52860 Alkyne?CPG?modifier?500,?5?g 1650.00 19800 21?days
22260 Alkyne?Phosphoramidite,?5′-terminal,?250?mg 230.00 2760 in?stock
42260 Alkyne?Phosphoramidite,?5′-terminal,?1?g 450.00 5400 in?stock
62260 Alkyne?Phosphoramidite,?5′-terminal,?5?g 1990.00 23880 10?days
15040 Amino-11-ddUTP,?1?umol 95.00 1140 in?stock
25040 Amino-11-ddUTP,?5?umol 195.00 2340 in?stock
45040 Amino-11-ddUTP,?25?umol 395.00 4740 in?stock
16040 Amino-11-dUTP,?1?umol 95.00 1140 in?stock
26040 Amino-11-dUTP,?5?umol 195.00 2340 in?stock
46040 Amino-11-dUTP,?25?umol 395.00 4740 in?stock
12050 Ascorbic?acid,?10?mg 80.00 960 in?stock
33720 Azidobutyric?acid?NHS?ester,?10?mg 115.00 1380 in?stock
43720 Azidobutyric?acid?NHS?ester,?25?mg 195.00 2340 in?stock
53720 Azidobutyric?acid?NHS?ester,?50?mg 295.00 3540 in?stock
11430 BDP?FL?azide,?1?mg 110.00 1320 in?stock
21430 BDP?FL?azide,?5?mg 210.00 2520 in?stock
31430 BDP?FL?azide,?10?mg 310.00 3720 in?stock
41430 BDP?FL?azide,?25?mg 410.00 4920 in?stock
51430 BDP?FL?azide,?50?mg 695.00 8340 in?stock
11480 BDP?FL?maleimide,?1?mg 110.00 1320 in?stock
21480 BDP?FL?maleimide,?5?mg 210.00 2520 in?stock
41480 BDP?FL?maleimide,?25?mg 410.00 4920 in?stock
51480 BDP?FL?maleimide,?50?mg 695.00 8340 in?stock
61480 BDP?FL?maleimide,?100?mg 1190.00 14280 in?stock
11420 BDP?FL?NHS?ester,?1?mg 110.00 1320 in?stock
21420 BDP?FL?NHS?ester,?5?mg 210.00 2520 in?stock
41420 BDP?FL?NHS?ester,?25?mg 410.00 4920 in?stock
51420 BDP?FL?NHS?ester,?50?mg 695.00 8340 in?stock
61420 BDP?FL?NHS?ester,?100?mg 1190.00 14280 in?stock
C37B0 Biotin?alkyne,?10?mg 90.00 1080 in?stock
D37B0 Biotin?alkyne,?25?mg 160.00 1920 in?stock
E37B0 Biotin?alkyne,?50?mg 270.00 3240 in?stock
F37B0 Biotin?alkyne,?100?mg 420.00 5040 in?stock
C3730 Biotin?azide,?10?mg 90.00 1080 in?stock
D3730 Biotin?azide,?25?mg 160.00 1920 in?stock
E3730 Biotin?azide,?50?mg 270.00 3240 in?stock
F3730 Biotin?azide,?100?mg 420.00 5040 in?stock
21050 Copper(II)-TBTA?complex,?10?mM?in?55%?aq.?DMSO,?1.5?mL 55.00 660 5?days
A1430 Coumarin?343?azide,?1?mg 110.00 1320 in?stock
B1430 Coumarin?343?azide,?5?mg 210.00 2520 in?stock
C1430 Coumarin?343?azide,?10?mg 310.00 3720 in?stock
D1430 Coumarin?343?azide,?25?mg 410.00 4920 in?stock
E1430 Coumarin?343?azide,?50?mg 695.00 8340 21?days
1A041 Cyanine2?NHS?ester?minimal?dye,?5?nmol 85.00 1020 in?stock
2A041 Cyanine2?NHS?ester?minimal?dye,?10?nmol 140.00 1680 in?stock
3A041 Cyanine2?NHS?ester?minimal?dye,?25?nmol 210.00 2520 in?stock
A10B0 Cyanine3?alkyne,?1?mg 110.00 1320 in?stock
B10B0 Cyanine3?alkyne,?5?mg 210.00 2520 in?stock
C10B0 Cyanine3?alkyne,?10?mg 310.00 3720 in?stock
D10B0 Cyanine3?alkyne,?25?mg 410.00 4920 in?stock
E10B0 Cyanine3?alkyne,?50?mg 695.00 8340 in?stock
110C0 Cyanine3?amine,?1?mg 110.00 1320 in?stock
210C0 Cyanine3?amine,?5?mg 210.00 2520 in?stock
410C0 Cyanine3?amine,?25?mg 410.00 4920 in?stock
510C0 Cyanine3?amine,?50?mg 695.00 8340 in?stock
610C0 Cyanine3?amine,?100?mg 1190.00 14280 in?stock
11030 Cyanine3?azide,?100?uL,?10?mM/DMSO 110.00 1320 in?stock
31030 Cyanine3?azide,?500?uL,?10?mM/DMSO 210.00 2520 in?stock
41030 Cyanine3?azide,?1?mL,?10?mM/DMSO 410.00 4920 in?stock
A1030 Cyanine3?azide,?1?mg 110.00 1320 in?stock
B1030 Cyanine3?azide,?5?mg 210.00 2520 in?stock
C1030 Cyanine3?azide,?10?mg 310.00 3720 in?stock
D1030 Cyanine3?azide,?25?mg 410.00 4920 in?stock
E1030 Cyanine3?azide,?50?mg 695.00 8340 in?stock
11090 Cyanine3?carboxylic?acid,?1?mg 110.00 1320 in?stock
21090 Cyanine3?carboxylic?acid,?5?mg 210.00 2520 in?stock
41090 Cyanine3?carboxylic?acid,?25?mg 410.00 4920 in?stock
51090 Cyanine3?carboxylic?acid,?50?mg 695.00 8340 in?stock
61090 Cyanine3?carboxylic?acid,?100?mg 1190.00 14280 in?stock
11070 Cyanine3?hydrazide,?1?mg 110.00 1320 in?stock
21070 Cyanine3?hydrazide,?5?mg 210.00 2520 in?stock
41070 Cyanine3?hydrazide,?25?mg 410.00 4920 in?stock
51070 Cyanine3?hydrazide,?50?mg 695.00 8340 in?stock
61070 Cyanine3?hydrazide,?100?mg 1190.00 14280 in?stock
11080 Cyanine3?maleimide,?1?mg 110.00 1320 in?stock
21080 Cyanine3?maleimide,?5?mg 210.00 2520 in?stock
41080 Cyanine3?maleimide,?25?mg 410.00 4920 in?stock
51080 Cyanine3?maleimide,?50?mg 695.00 8340 in?stock
61080 Cyanine3?maleimide,?100?mg 1190.00 14280 in?stock
11020 Cyanine3?NHS?ester,?1?mg 110.00 1320 in?stock
21020 Cyanine3?NHS?ester,?5?mg 210.00 2520 in?stock
41020 Cyanine3?NHS?ester,?25?mg 410.00 4920 in?stock
51020 Cyanine3?NHS?ester,?50?mg 695.00 8340 in?stock
61020 Cyanine3?NHS?ester,?100?mg 1190.00 14280 in?stock
1B041 Cyanine3?NHS?ester?minimal?dye,?5?nmol 85.00 1020 in?stock
2B041 Cyanine3?NHS?ester?minimal?dye,?10?nmol 140.00 1680 in?stock
3B041 Cyanine3?NHS?ester?minimal?dye,?25?nmol 210.00 2520 in?stock
12030 Cyanine3.5?azide,?100?uL,?10?mM/DMSO 110.00 1320 in?stock
32030 Cyanine3.5?azide,?500?uL,?10?mM/DMSO 210.00 2520 in?stock
42030 Cyanine3.5?azide,?1?mL,?10?mM/DMSO 410.00 4920 in?stock
A2030 Cyanine3.5?azide,?1?mg 110.00 1320 in?stock
B2030 Cyanine3.5?azide,?5?mg 210.00 2520 in?stock
C2030 Cyanine3.5?azide,?10?mg 310.00 3720 in?stock
D2030 Cyanine3.5?azide,?25?mg 410.00 4920 in?stock
E2030 Cyanine3.5?azide,?50?mg 695.00 8340 in?stock
12090 Cyanine3.5?carboxylic?acid,?1?mg 110.00 1320 in?stock
22090 Cyanine3.5?carboxylic?acid,?5?mg 210.00 2520 in?stock
42090 Cyanine3.5?carboxylic?acid,?25?mg 410.00 4920 in?stock
52090 Cyanine3.5?carboxylic?acid,?50?mg 695.00 8340 in?stock
62090 Cyanine3.5?carboxylic?acid,?100?mg 1190.00 14280 in?stock
12020 Cyanine3.5?NHS?ester,?1?mg 110.00 1320 in?stock
22020 Cyanine3.5?NHS?ester,?5?mg 210.00 2520 in?stock
42020 Cyanine3.5?NHS?ester,?25?mg 410.00 4920 in?stock
52020 Cyanine3.5?NHS?ester,?50?mg 695.00 8340 in?stock
62020 Cyanine3.5?NHS?ester,?100?mg 1190.00 14280 in?stock
A30B0 Cyanine5?alkyne,?1?mg 110.00 1320 in?stock
B30B0 Cyanine5?alkyne,?5?mg 210.00 2520 in?stock
C30B0 Cyanine5?alkyne,?10?mg 310.00 3720 in?stock
D30B0 Cyanine5?alkyne,?25?mg 410.00 4920 in?stock
E30B0 Cyanine5?alkyne,?50?mg 695.00 8340 in?stock
130C0 Cyanine5?amine,?1?mg 110.00 1320 in?stock
230C0 Cyanine5?amine,?5?mg 210.00 2520 in?stock
430C0 Cyanine5?amine,?25?mg 410.00 4920 5?days
530C0 Cyanine5?amine,?50?mg 695.00 8340 5?days
630C0 Cyanine5?amine,?100?mg 1190.00 14280 in?stock
13030 Cyanine5?azide,?100?uL,?10?mM/DMSO 110.00 1320 in?stock
33030 Cyanine5?azide,?500?uL,?10?mM/DMSO 210.00 2520 in?stock
43030 Cyanine5?azide,?1?mL,?10?mM/DMSO 410.00 4920 in?stock
A3030 Cyanine5?azide,?1?mg 110.00 1320 in?stock
B3030 Cyanine5?azide,?5?mg 210.00 2520 in?stock
C3030 Cyanine5?azide,?10?mg 310.00 3720 in?stock
D3030 Cyanine5?azide,?25?mg 410.00 4920 in?stock
E3030 Cyanine5?azide,?50?mg 695.00 8340 5?days
130A0 Cyanine5?Boc-hydrazide,?1?mg 110.00 1320 21?days
230A0 Cyanine5?Boc-hydrazide,?5?mg 210.00 2520 21?days
430A0 Cyanine5?Boc-hydrazide,?25?mg 410.00 4920 21?days
530A0 Cyanine5?Boc-hydrazide,?50?mg 695.00 8340 21?days
630A0 Cyanine5?Boc-hydrazide,?100?mg 1190.00 14280 21?days
13090 Cyanine5?carboxylic?acid,?1?mg 110.00 1320 in?stock
23090 Cyanine5?carboxylic?acid,?5?mg 210.00 2520 in?stock
43090 Cyanine5?carboxylic?acid,?25?mg 410.00 4920 in?stock
53090 Cyanine5?carboxylic?acid,?50?mg 695.00 8340 in?stock
63090 Cyanine5?carboxylic?acid,?100?mg 1190.00 14280 in?stock
13070 Cyanine5?hydrazide,?1?mg 110.00 1320 in?stock
23070 Cyanine5?hydrazide,?5?mg 210.00 2520 in?stock
43070 Cyanine5?hydrazide,?25?mg 410.00 4920 in?stock
53070 Cyanine5?hydrazide,?50?mg 695.00 8340 in?stock
63070 Cyanine5?hydrazide,?100?mg 1190.00 14280 in?stock
13080 Cyanine5?maleimide,?1?mg 110.00 1320 in?stock
23080 Cyanine5?maleimide,?5?mg 210.00 2520 in?stock
43080 Cyanine5?maleimide,?25?mg 410.00 4920 in?stock
53080 Cyanine5?maleimide,?50?mg 695.00 8340 in?stock
63080 Cyanine5?maleimide,?100?mg 1190.00 14280 in?stock
13020 Cyanine5?NHS?ester,?1?mg 110.00 1320 in?stock
23020 Cyanine5?NHS?ester,?5?mg 210.00 2520 in?stock
43020 Cyanine5?NHS?ester,?25?mg 410.00 4920 in?stock
53020 Cyanine5?NHS?ester,?50?mg 695.00 8340 in?stock
63020 Cyanine5?NHS?ester,?100?mg 1190.00 14280 in?stock
1C041 Cyanine5?NHS?ester?minimal?dye,?5?nmol 85.00 1020 in?stock
2C041 Cyanine5?NHS?ester?minimal?dye,?10?nmol 140.00 1680 in?stock
3C041 Cyanine5?NHS?ester?minimal?dye,?25?nmol 210.00 2520 in?stock
A70B0 Cyanine5.5?alkyne,?1?mg 110.00 1320 in?stock
B70B0 Cyanine5.5?alkyne,?5?mg 210.00 2520 in?stock
C70B0 Cyanine5.5?alkyne,?10?mg 310.00 3720 in?stock
D70B0 Cyanine5.5?alkyne,?25?mg 410.00 4920 in?stock
E70B0 Cyanine5.5?alkyne,?50?mg 695.00 8340 in?stock
170C0 Cyanine5.5?amine,?1?mg 110.00 1320 in?stock
270C0 Cyanine5.5?amine,?5?mg 210.00 2520 in?stock
470C0 Cyanine5.5?amine,?25?mg 210.00 2520 in?stock
570C0 Cyanine5.5?amine,?50?mg 695.00 8340 in?stock
670C0 Cyanine5.5?amine,?100?mg 1190.00 14280 in?stock
14030 Cyanine5.5?azide,?100?uL,?10?mM/DMSO 110.00 1320 in?stock
34030 Cyanine5.5?azide,?500?uL,?10?mM/DMSO 210.00 2520 in?stock
44030 Cyanine5.5?azide,?1?mL,?10?mM/DMSO 410.00 4920 in?stock
A4030 Cyanine5.5?azide,?1?mg 110.00 1320 in?stock
B4030 Cyanine5.5?azide,?5?mg 210.00 2520 in?stock
C4030 Cyanine5.5?azide,?10?mg 310.00 3720 in?stock
D4030 Cyanine5.5?azide,?25?mg 410.00 4920 in?stock
E4030 Cyanine5.5?azide,?50?mg 695.00 8340 in?stock
17090 Cyanine5.5?carboxylic?acid,?1?mg 110.00 1320 in?stock
27090 Cyanine5.5?carboxylic?acid,?5?mg 210.00 2520 in?stock
47090 Cyanine5.5?carboxylic?acid,?25?mg 410.00 4920 in?stock
57090 Cyanine5.5?carboxylic?acid,?50?mg 695.00 8340 in?stock
67090 Cyanine5.5?carboxylic?acid,?100?mg 1190.00 14280 in?stock
17070 Cyanine5.5?hydrazide,?1?mg 110.00 1320 in?stock
27070 Cyanine5.5?hydrazide,?5?mg 210.00 2520 in?stock
47070 Cyanine5.5?hydrazide,?25?mg 410.00 4920 in?stock
57070 Cyanine5.5?hydrazide,?50?mg 695.00 8340 in?stock
67070 Cyanine5.5?hydrazide,?100?mg 1190.00 14280 in?stock
17080 Cyanine5.5?maleimide,?1?mg 110.00 1320 in?stock
27080 Cyanine5.5?maleimide,?5?mg 210.00 2520 in?stock
47080 Cyanine5.5?maleimide,?25?mg 410.00 4920 in?stock
57080 Cyanine5.5?maleimide,?50?mg 695.00 8340 in?stock
67080 Cyanine5.5?maleimide,?100?mg 1190.00 14280 5?days
17020 Cyanine5.5?NHS?ester,?1?mg 110.00 1320 in?stock
27020 Cyanine5.5?NHS?ester,?5?mg 210.00 2520 in?stock
47020 Cyanine5.5?NHS?ester,?25?mg 410.00 4920 in?stock
57020 Cyanine5.5?NHS?ester,?50?mg 695.00 8340 in?stock
67020 Cyanine5.5?NHS?ester,?100?mg 1190.00 14280 in?stock
150C0 Cyanine7?amine,?1?mg 110.00 1320 in?stock
250C0 Cyanine7?amine,?5?mg 210.00 2520 in?stock
450C0 Cyanine7?amine,?25?mg 410.00 4920 in?stock
550C0 Cyanine7?amine,?50?mg 695.00 8340 in?stock
650C0 Cyanine7?amine,?100?mg 1190.00 14280 in?stock
15030 Cyanine7?azide,?100?uL,?10?mM/DMSO 110.00 1320 in?stock
35030 Cyanine7?azide,?500?uL,?10?mM/DMSO 210.00 2520 in?stock
45030 Cyanine7?azide,?1?mL,?10?mM/DMSO 410.00 4920 in?stock
A5030 Cyanine7?azide,?1?mg 110.00 1320 in?stock
B5030 Cyanine7?azide,?5?mg 210.00 2520 in?stock
C5030 Cyanine7?azide,?10?mg 310.00 3720 in?stock
D5030 Cyanine7?azide,?25?mg 410.00 4920 in?stock
E5030 Cyanine7?azide,?50?mg 695.00 8340 in?stock
15090 Cyanine7?carboxylic?acid,?1?mg 110.00 1320 in?stock
25090 Cyanine7?carboxylic?acid,?5?mg 210.00 2520 in?stock
45090 Cyanine7?carboxylic?acid,?25?mg 410.00 4920 in?stock
55090 Cyanine7?carboxylic?acid,?50?mg 695.00 8340 in?stock
65090 Cyanine7?carboxylic?acid,?100?mg 1190.00 14280 in?stock
15080 Cyanine7?maleimide,?1?mg 110.00 1320 in?stock
25080 Cyanine7?maleimide,?5?mg 210.00 2520 in?stock
45080 Cyanine7?maleimide,?25?mg 410.00 4920 in?stock
55080 Cyanine7?maleimide,?50?mg 695.00 8340 in?stock
65080 Cyanine7?maleimide,?100?mg 1190.00 14280 in?stock
15020 Cyanine7?NHS?ester,?1?mg 110.00 1320 in?stock
25020 Cyanine7?NHS?ester,?5?mg 210.00 2520 in?stock
45020 Cyanine7?NHS?ester,?25?mg 410.00 4920 in?stock
55020 Cyanine7?NHS?ester,?50?mg 695.00 8340 in?stock
65020 Cyanine7?NHS?ester,?100?mg 1190.00 14280 in?stock
160C0 Cyanine7.5?amine,?1?mg 110.00 1320 in?stock
260C0 Cyanine7.5?amine,?5?mg 210.00 2520 in?stock
460C0 Cyanine7.5?amine,?25?mg 410.00 4920 in?stock
560C0 Cyanine7.5?amine,?50?mg 695.00 8340 in?stock
660C0 Cyanine7.5?amine,?100?mg 1190.00 14280 in?stock
16030 Cyanine7.5?azide,?100?uL,?10?mM?in?DMS 110.00 1320 in?stock
36030 Cyanine7.5?azide,?500?uL,?10?mM?in?DMS 210.00 2520 in?stock
46030 Cyanine7.5?azide,?1?mL,?10?mM?in?DMSO 410.00 4920 in?stock
A6030 Cyanine7.5?azide,?1?mg 110.00 1320 in?stock
B6030 Cyanine7.5?azide,?5?mg 210.00 2520 in?stock
C6030 Cyanine7.5?azide,?10?mg 310.00 3720 in?stock
D6030 Cyanine7.5?azide,?25?mg 410.00 4920 in?stock
E6030 Cyanine7.5?azide,?50?mg 695.00 8340 in?stock
16090 Cyanine7.5?carboxylic?acid,?1?mg 110.00 1320 in?stock
26090 Cyanine7.5?carboxylic?acid,?5?mg 210.00 2520 in?stock
46090 Cyanine7.5?carboxylic?acid,?25?mg 410.00 4920 in?stock
56090 Cyanine7.5?carboxylic?acid,?50?mg 695.00 8340 in?stock
66090 Cyanine7.5?carboxylic?acid,?100?mg 1190.00 14280 in?stock
16080 Cyanine7.5?maleimide,?1?mg 110.00 1320 in?stock
26080 Cyanine7.5?maleimide,?5?mg 210.00 2520 in?stock
46080 Cyanine7.5?maleimide,?25?mg 410.00 4920 in?stock
56080 Cyanine7.5?maleimide,?50?mg 695.00 8340 in?stock
66080 Cyanine7.5?maleimide,?100?mg 1190.00 14280 in?stock
16020 Cyanine7.5?NHS?ester,?1?mg 110.00 1320 in?stock
26020 Cyanine7.5?NHS?ester,?5?mg 210.00 2520 5?days
46020 Cyanine7.5?NHS?ester,?25?mg 410.00 4920 in?stock
56020 Cyanine7.5?NHS?ester,?50?mg 695.00 8340 in?stock
66020 Cyanine7.5?NHS?ester,?100?mg 1190.00 14280 in?stock
13050 DMF?(dimethylformamide),?labeling?grade,?1?mL 35.00 420 in?stock
23260 DMS(O)MT?aminolink?C6,?250?mg 200.00 2400 10?days
43260 DMS(O)MT?aminolink?C6,?1?g 420.00 5040 10?days
63260 DMS(O)MT?aminolink?C6,?5?g 1450.00 17400 21?days
A41B0 FAM?alkyne,?5-isomer,?1?mg 80.00 960 in?stock
B41B0 FAM?alkyne,?5-isomer,?5?mg 100.00 1200 in?stock
C41B0 FAM?alkyne,?5-isomer,?10?mg 150.00 1800 5?days
D41B0 FAM?alkyne,?5-isomer,?25?mg 295.00 3540 in?stock
E41B0 FAM?alkyne,?5-isomer,?50?mg 450.00 5400 in?stock
14130 FAM?azide,?5-isomer,?100?uL,?10?mM/DMSO 80.00 960 in?stock
34130 FAM?azide,?5-isomer,?500?uL,?10?mM/DMSO 100.00 1200 in?stock
44130 FAM?azide,?5-isomer,?1?mL,?10?mM/DMSO 150.00 1800 in?stock
A4130 FAM?azide,?5-isomer,?1?mg 80.00 960 in?stock
B4130 FAM?azide,?5-isomer,?5?mg 100.00 1200 in?stock
C4130 FAM?azide,?5-isomer,?10?mg 150.00 1800 in?stock
D4130 FAM?azide,?5-isomer,?25?mg 295.00 3540 in?stock
E4130 FAM?azide,?5-isomer,?50?mg 450.00 5400 in?stock
15130 FAM?azide,?6-isomer,?100?uL,?10?mM/DMSO 80.00 960 in?stock
35130 FAM?azide,?6-isomer,?500?uL,?10?mM/DMSO 100.00 1200 in?stock
45130 FAM?azide,?6-isomer,?1?mL,?10?mM/DMSO 150.00 1800 in?stock
A5130 FAM?azide,?6-isomer,?1?mg 80.00 960 in?stock
B5130 FAM?azide,?6-isomer,?5?mg 100.00 1200 in?stock
C5130 FAM?azide,?6-isomer,?10?mg 150.00 1800 in?stock
D5130 FAM?azide,?6-isomer,?25?mg 295.00 3540 in?stock
E5130 FAM?azide,?6-isomer,?50?mg 450.00 5400 in?stock
C5160 FAM?Phosphoramidite,?6-Isomer,?100?umol?(85?mg) 75.00 900 in?stock
D5160 FAM?Phosphoramidite,?6-Isomer,?250?mg 150.00 1800 in?stock
F5160 FAM?Phosphoramidite,?6-Isomer,?1?g 450.00 5400 5?days
G5160 FAM?Phosphoramidite,?6-Isomer,?5?g 1900.00 22800 10?days
24260 Long?trebler?phosphoramidite,?250?mg 250.00 3000 10?days
34260 Long?trebler?phosphoramidite,?500?mg 350.00 4200 in?stock
44260 Long?trebler?phosphoramidite,?1?g 600.00 7200 21?days
30720 Pentynoic?acid?STP?ester,?10?mg 110.00 1320 in?stock
40720 Pentynoic?acid?STP?ester,?25?mg 210.00 2520 in?stock
50720 Pentynoic?acid?STP?ester,?50?mg 410.00 4920 5?days
60720 Pentynoic?acid?STP?ester,?100?mg 695.00 8340 in?stock
A4530 PEP?azide,?1?mg 110.00 1320 in?stock
B4530 PEP?azide,?5?mg 210.00 2520 in?stock
C4530 PEP?azide,?10?mg 310.00 3720 in?stock
D4530 PEP?azide,?25?mg 410.00 4920 in?stock
E4530 PEP?azide,?50?mg 695.00 8340 in?stock
A5530 Perylene?azide,?1?mg 110.00 1320 5?days
B5530 Perylene?azide,?5?mg 210.00 2520 in?stock
C5530 Perylene?azide,?10?mg 310.00 3720 in?stock
D5530 Perylene?azide,?25?mg 410.00 4920 5?days
E5530 Perylene?azide,?50?mg 695.00 8340 in?stock
A1530 Pyrene?azide?1,?1?mg 110.00 1320 in?stock
B1530 Pyrene?azide?1,?5?mg 210.00 2520 in?stock
C1530 Pyrene?azide?1,?10?mg 310.00 3720 in?stock
D1530 Pyrene?azide?1,?25?mg 410.00 4920 in?stock
E1530 Pyrene?azide?1,?50?mg 695.00 8340 in?stock
A2530 Pyrene?azide?2,?1?mg 110.00 1320 in?stock
B2530 Pyrene?azide?2,?5?mg 210.00 2520 in?stock
C2530 Pyrene?azide?2,?10?mg 310.00 3720 in?stock
D2530 Pyrene?azide?2,?25?mg 410.00 4920 in?stock
E2530 Pyrene?azide?2,?50?mg 695.00 8340 in?stock
A3530 Pyrene?azide?3,?1?mg 110.00 1320 5?days
B3530 Pyrene?azide?3,?5?mg 210.00 2520 5?days
C3530 Pyrene?azide?3,?10?mg 310.00 3720 in?stock
D3530 Pyrene?azide?3,?25?mg 410.00 4920 5?days
E3530 Pyrene?azide?3,?50?mg 695.00 8340 21?days
11460 Pyrene?phosphoramidite?dU,?100?mg 150.00 1800 in?stock
21460 Pyrene?phosphoramidite?dU,?250?mg 320.00 3840 10?days
41460 Pyrene?phosphoramidite?dU,?1?g 880.00 10560 10?days
11230 ROX?azide,?5-?isomer,?100?uL,?10?mM/DMSO 110.00 1320 in?stock
31230 ROX?azide,?5-?isomer,?500?uL,?10?mM/DMSO 210.00 2520 in?stock
41230 ROX?azide,?5-?isomer,?1?mL,?10?mM/DMSO 410.00 4920 in?stock
A1230 ROX?azide,?5-?isomer,?1?mg 110.00 1320 in?stock
B1230 ROX?azide,?5-?isomer,?5?mg 210.00 2520 in?stock
C1230 ROX?azide,?5-?isomer,?10?mg 310.00 3720 in?stock
D1230 ROX?azide,?5-?isomer,?25?mg 410.00 4920 in?stock
E1230 ROX?azide,?5-?isomer,?50?mg 695.00 8340 in?stock
12220 ROX?NHS?ester,?pure?6-?isomer,?1?mg 110.00 1320 in?stock
22220 ROX?NHS?ester,?pure?6-?isomer,?5?mg 210.00 2520 in?stock
42220 ROX?NHS?ester,?pure?6-?isomer,?25?mg 410.00 4920 in?stock
52220 ROX?NHS?ester,?pure?6-?isomer,?50?mg 695.00 8340 in?stock
62220 ROX?NHS?ester,?pure?6-?isomer,?100?mg 1190.00 14280 in?stock
31110 ROX?reference?dye?for?qPCR,?500?uL 30.00 360 21?days
A1330 Sulfo-Cyanine3?azide,?1?mg 160.00 1920 in?stock
B1330 Sulfo-Cyanine3?azide,?5?mg 410.00 4920 in?stock
C1330 Sulfo-Cyanine3?azide,?10?mg 595.00 7140 in?stock
D1330 Sulfo-Cyanine3?azide,?25?mg 850.00 10200 in?stock
11390 Sulfo-Cyanine3?carboxylic?acid,?1?mg 160.00 1920 in?stock
21390 Sulfo-Cyanine3?carboxylic?acid,?5?mg 410.00 4920 in?stock
41390 Sulfo-Cyanine3?carboxylic?acid,?25?mg 850.00 10200 in?stock
51390 Sulfo-Cyanine3?carboxylic?acid,?50?mg 1500.00 18000 in?stock
61390 Sulfo-Cyanine3?carboxylic?acid,?100?mg 2450.00 29400 in?stock
11380 Sulfo-Cyanine3?maleimide,?1?mg 160.00 1920 5?days
21380 Sulfo-Cyanine3?maleimide,?5?mg 410.00 4920 in?stock
41380 Sulfo-Cyanine3?maleimide,?25?mg 850.00 10200 in?stock
51380 Sulfo-Cyanine3?maleimide,?50?mg 1500.00 18000 in?stock
61380 Sulfo-Cyanine3?maleimide,?100?mg 2450.00 29400 in?stock
11320 Sulfo-Cyanine3?NHS?ester,?1?mg 160.00 1920 in?stock
21320 Sulfo-Cyanine3?NHS?ester,?5?mg 410.00 4920 in?stock
41320 Sulfo-Cyanine3?NHS?ester,?25?mg 850.00 10200 in?stock
51320 Sulfo-Cyanine3?NHS?ester,?50?mg 1500.00 18000 in?stock
61320 Sulfo-Cyanine3?NHS?ester,?100?mg 2450.00 29400 in?stock
A33B0 Sulfo-Cyanine5?alkyne,?1?mg 160.00 1920 in?stock
B33B0 Sulfo-Cyanine5?alkyne,?5?mg 410.00 4920 in?stock
C33B0 Sulfo-Cyanine5?alkyne,?10?mg 595.00 7140 in?stock
D33B0 Sulfo-Cyanine5?alkyne,?25?mg 850.00 10200 in?stock
A3330 Sulfo-Cyanine5?azide,?1?mg 160.00 1920 in?stock
B3330 Sulfo-Cyanine5?azide,?5?mg 410.00 4920 in?stock
C3330 Sulfo-Cyanine5?azide,?10?mg 595.00 7140 in?stock
D3330 Sulfo-Cyanine5?azide,?25?mg 850.00 10200 in?stock
13390 Sulfo-Cyanine5?carboxylic?acid,?1?mg 160.00 1920 in?stock
23390 Sulfo-Cyanine5?carboxylic?acid,?5?mg 410.00 4920 in?stock
43390 Sulfo-Cyanine5?carboxylic?acid,?25?mg 850.00 10200 in?stock
53390 Sulfo-Cyanine5?carboxylic?acid,?50?mg 1500.00 18000 in?stock
63390 Sulfo-Cyanine5?carboxylic?acid,?100?mg 2450.00 29400 in?stock
13380 Sulfo-Cyanine5?maleimide,?1?mg 160.00 1920 21?days
23380 Sulfo-Cyanine5?maleimide,?5?mg 410.00 4920 in?stock
43380 Sulfo-Cyanine5?maleimide,?25?mg 850.00 10200 in?stock
53380 Sulfo-Cyanine5?maleimide,?50?mg 1500.00 18000 in?stock
63380 Sulfo-Cyanine5?maleimide,?100?mg 2450.00 29400 in?stock
13320 Sulfo-Cyanine5?NHS?ester,?1?mg 160.00 1920 in?stock
23320 Sulfo-Cyanine5?NHS?ester,?5?mg 410.00 4920 in?stock
43320 Sulfo-Cyanine5?NHS?ester,?25?mg 850.00 10200 in?stock
53320 Sulfo-Cyanine5?NHS?ester,?50?mg 1500.00 18000 in?stock
63320 Sulfo-Cyanine5?NHS?ester,?100?mg 2450.00 29400 in?stock
153C0 Sulfo-Cyanine7?amine,?1?mg 160.00 1920 in?stock
253C0 Sulfo-Cyanine7?amine,?5?mg 410.00 4920 in?stock
453C0 Sulfo-Cyanine7?amine,?25?mg 850.00 10200 in?stock
553C0 Sulfo-Cyanine7?amine,?50?mg 1500.00 18000 in?stock
653C0 Sulfo-Cyanine7?amine,?100?mg 2450.00 29400 5?days
A5330 Sulfo-Cyanine7?azide,?1?mg 160.00 1920 in?stock
B5330 Sulfo-Cyanine7?azide,?5?mg 410.00 4920 in?stock
C5330 Sulfo-Cyanine7?azide,?10?mg 595.00 7140 in?stock
D5330 Sulfo-Cyanine7?azide,?25?mg 850.00 10200 in?stock
15390 Sulfo-Cyanine7?carboxylic?acid,?1?mg 160.00 1920 in?stock
25390 Sulfo-Cyanine7?carboxylic?acid,?5?mg 410.00 4920 in?stock
45390 Sulfo-Cyanine7?carboxylic?acid,?25?mg 850.00 10200 in?stock
55390 Sulfo-Cyanine7?carboxylic?acid,?50?mg 1500.00 18000 in?stock
65390 Sulfo-Cyanine7?carboxylic?acid,?100?mg 2450.00 29400 in?stock
15320 Sulfo-Cyanine7?NHS?ester,?1?mg 160.00 1920 in?stock
25320 Sulfo-Cyanine7?NHS?ester,?5?mg 410.00 4920 in?stock
45320 Sulfo-Cyanine7?NHS?ester,?25?mg 850.00 10200 in?stock
55320 Sulfo-Cyanine7?NHS?ester,?50?mg 1500.00 18000 in?stock
65320 Sulfo-Cyanine7?NHS?ester,?100?mg 2450.00 29400 in?stock
41010 SYBR?Green?I?for?Real?Time?PCR,?100x,?1?mL 310.00 3720 in?stock
51010 SYBR?Green?I?for?Real?Time?PCR,?100x,?1.5?mL 450.00 5400 in?stock
51010-5 SYBR?Green?I?for?Real?Time?PCR,?100x,?5×1.5?mL 750.00 9000 10?days
20010 SYBR?Green?I?Gel?Staining?Solution,?10000x,?1?mL 180.00 2160 in?stock
17130 TAMRA?azide,?5-?isomer,?100?uL,?10?mM/DMSO 110.00 1320 in?stock
37130 TAMRA?azide,?5-?isomer,?500?uL,?10?mM/DMSO 210.00 2520 in?stock
47130 TAMRA?azide,?5-?isomer,?1?mL,?10?mM/DMSO 410.00 4920 in?stock
A7130 TAMRA?azide,?5-?isomer,?1?mg 110.00 1320 in?stock
B7130 TAMRA?azide,?5-?isomer,?5?mg 210.00 2520 in?stock
C7130 TAMRA?azide,?5-?isomer,?10?mg 310.00 3720 in?stock
D7130 TAMRA?azide,?5-?isomer,?25?mg 410.00 4920 in?stock
E7130 TAMRA?azide,?5-?isomer,?50?mg 695.00 8340 in?stock
21860 TFA-Amino?modifier?CPG?500,?250?mg 120.00 1440 10?days
41860 TFA-Amino?modifier?CPG?500,?1?g 380.00 4560 10?days
51860 TFA-Amino?modifier?CPG?500,?5?g 1650.00 19800 21?days

D-果糖/D-葡萄糖[液體即用型]檢測試劑盒 D-Fructose /D-Glucose (Liquid Ready Reagent) Assay Kit 貨號:K-FRGLQR Megazyme中文站

D-果糖/D-葡萄糖[液體即用型]檢測試劑盒

英文名:D-Fructose /D-Glucose (Liquid Ready Reagent) Assay Kit

貨號:K-FRGLQR

規格:1100 assays per kit in autoanalyser format

The D-Fructose/D-Glucose (Liquid Ready Reagents) test kit is a rapid, reliable and accurate method for the specific measurement and analysis of D-fructose and D-glucose in wine, beverages, foodstuffs and other materials. Supplied as a "ready to use" liquid stable formulation that is suitable for auto-analyser and microplate formats.
Suitable for auto-analyser and microplate formats.

UV-method suitable for auto-analyser and microplate formats for
the determination of D-Fructose and D-Glucose in foodstuffs,
beverages and other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphoglucose isomerase)
(4) F-6-P ↔ G-6-P

Kit size: 1100 assays (microplate) / 1100 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 13 min
Detection limit: 133 mg/L (recommended format)
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods,
bread, bakery products, candies, desserts, confectionery, ice-cream,
fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals,
paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

Advantages

  • PVP incorporated to prevent tannin inhibition
  • “Ready to use” liquid stable formulation
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years
  • Very rapid reaction (~ 13 min)
  • Standard included
  • Suitable for microplate and auto-analyser formats

 Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q5. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q6. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q7. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q8. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.

Q9. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q10. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q11. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q12. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q13. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q14. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q15. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q16. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

果聚糖檢測試劑盒 Fructan Assay Kit 貨號:K-FRUC Megazyme中文站

果聚糖檢測試劑盒

英文名:Fructan Assay Kit

貨號:K-FRUC

規格:100 assays per kit

產品名稱:果聚糖檢測試劑盒
英文名稱:Fructan Assay Kit
型號規格:100次
品牌:愛爾蘭Megaeyme公司

果聚糖廣泛分布于植物界。單子葉植物,雙子葉植物和綠藻中均存在。

以分子結構和分子重量區別果聚糖。也可以分為三類:菊粉組,果聚糖組和帶支鏈組。菊粉組主要或專門由果糖基果糖鍵組成。果聚糖組主要或專門由果糖基果糖鍵組成。帶支鏈組由大量的(2→1)和(2→6)果糖基果糖鍵共同組成。

目前存在很多測定植物材料和食品中果聚糖的方法。被廣泛接受的是水解為D-果糖(和D-葡萄糖)后再測定的方法。這存在將蔗糖,D-果糖和D-葡萄糖分別去除或測定的問題,Pontis(1966)已經報道了蔗糖,D-葡萄糖和D-果糖的去除方法,利用蔗糖酶水解蔗糖,再通過氫氧化鈉煮沸破壞隨之產生的D-果糖和D-葡萄糖及原有的單糖。據報道,蔗糖酶對菊粉組低聚果糖的作用是緩慢的,可以通過選擇正確的孵育時間使其失效。通過測定目前使用的純化的酵母蔗糖酶,發現很難。

但是,本試劑盒提供的方法操作簡單,使用標準實驗室設備,并且是精確性,可重復性和專一性都很好。采用的酶是高純度酶,專一性水解蔗糖,淀粉和果聚糖。采用的蔗糖酶快速水解蔗糖但對1-蔗果三糖和其他果聚糖的水解活性可以忽略不計(McCleary和Blakeney,1999)(圖2)。在底物濃度為10 mg/mL時,蔗糖與1-蔗果三糖的相對水解比率為3,800:1。

原理:

蔗糖被蔗糖酶水解為D-葡萄糖和D-果糖。同時,如果樣品中存在淀粉和麥芽多糖通過高純β-淀粉酶,普魯蘭酶和麥芽糖酶聯合作用被水解D-葡萄糖。用堿性***物處理生成的還原糖,將其繼續被還原為糖醇。用稀釋的乙酸將溶液調至中性,并去除過量的***物。果聚糖被純化果聚糖酶(菊粉外切酶)水解為D-葡萄糖和D-果糖,通過PAHBAH法測定生成的還原糖。這個方法方便操作,D-葡萄糖和D-果糖的對應顏色一致。含有半乳糖苷蔗糖寡糖的樣品如(棉子糖)在蔗糖/淀粉酶混合物(酶溶液1)處理前推薦使用α-半乳糖苷酶(來源于黑曲霉)孵育。釋放的單糖通過堿性***物處理去除。如果處理中包括這一步驟,需要在計算中考慮體積的變化(如溶液5的最終體積應用1.15mL代替1.1mL).

 

分析物意義:許多食品如洋蔥和種子中的常見組分

Megazyme檢測試劑盒優點:方法新穎、反應快、試劑穩定 

The Fructan test kit is suitable for the specific measurement and analysis of fructan in plant extracts and food products containing starch, sucrose and other sugars.

Colourimetric method for the determination of Fructan in plant
products, foodstuffs and other materials

Principle:
(sucrase)
(1) Sucrose + H2O → D-glucose + D-fructose

(β-amylase + maltase + pullulanase)
(2) Starch + maltosaccharides + H2O → D-glucose

(borohydride)
(3) D-Glucose + D-fructose → D-sorbitol + D-mannitol
(non-reducing)

(exo-inulinase + endo-inulinase)
(4) Fructan + H2O → D-glucose + D-fructose

(100°C, 6 min)
(5) D-Glucose + D-fructose + PAHBAH → PAHBAH colour complex

Kit size: 100 assays
Method: Spectrophotometric at 410 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Flours, plant materials (e.g. onion), food products and other materials
Method recognition:
AOAC (Method 999.03), AACC (Method 32-32.01) and CODEX
(Type III Method)

Advantages

  • Very cost effective
  • All kit reagents stable for > 2 years after preparation
  • Unaffected by high sucrose / reducing sugar concentrations
  • Fructan kits are only available from Megazyme
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Does the fructanase enzyme hydrolyse Neosugar?

Yes.

Q3. When measuring fructan in starch containing samples, i.e. maize, is there a possibility of incomplete starch hydrolysis in step B?

From our experience, the level of beta-amylase/pullulanase etc. added is more than adequate to completely hydrolyse any starch likely to be present.  However, if there is a suspicion of incomplete starch hydrolysis, you can check this by running the hydrolysis with the pullulanase/beta-amylase etc. over a range of incubation times and comparing the results.

Q4. Could you tell me whether this method simply gives a gross measure of the fructan content, or whether it is possible to specifically determine the inulin content of the material?

The method measures inulin and cereal fructans.

Q5. What typical absorbance values are obtained for fructan control and fructose control?

Fructan Control Flour (29.6%) 0.496 0.498
Fructose Control 1.133 1.135.

Q6. What is the precision of the method for measurement of fructans?

Our reproducibility is usually +/- 5 to 7% of the fructan value.

Q7. Do you have statistics for the variability of your diagnostic test kits? I would like to get an idea what typical %RSD values would be for your oligofructan test kit.

With all our kits we can obtain approx. 5% c.v. in interlaboratory studies.  In-house these values are closer to 3%.

Q8. Can I detect the levan content of some plant materials by use of your fructan kit?

The fructanase in the kit will completely hydrolyse the fructans in plants.  These are either inulin type or "branched" type (as in oat or wheat stems).  We do not know if the enzymes would act on pure levan.

Q9. What actually is the factor value (F)? Is it always 54.5 or does it change depending on the fructose content?

The value of 54.5 is the actual amount of fructose standard used.  This amount of fructose will give a certain colour in the PAHBAH assay.
Thus; F = factor = [54.5 (micrograms of fructose)] divided by the absorbance value obtained for 54.5 micrograms of fructose in the PAHBAH method.

Q10. Is it possible to buy Fructanase separate from the kit?

Yes.  The best enzyme to use for analytical purposes is exo-Inulinase (E-EXOIAN).

Q11. We need to analyse sucrose in extracts of chicory roots and the sucrase used in your fructan assay procedure may solve the problem. Do you think that it would be possible to use sucrase instead of invertase in the sucrose assay?

Yes, the sugar enzyme can be used to measure sucrose.  The enzyme is an alpha-glucosidase, so it will also hydrolyse maltose if this is present.

Q12. Is it possible to do the weighing and hot extraction the day before the actual assay?

Extract solutions are best made and analysed the same day (not stored overnight). But if they are stored overnight in a refrigerator, heat them to 80?C, and cool before analysing (to ensure all fructan has re-dissolved).

Q13. Can the fructan method be used to measure inulin in starch-containing products, i.e. Cornflakes?

Yes.

Q14. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q15. Sucrase is specific for the cleavage of sucrose without hydrolysing any of the other potentially present oligo/polysaccharides (mixed linked glucan, soluble starch, fructan of any size & structure). Is this correct? Do you sell the enzyme separately?

Yes , the sucrase does hydrolyse sucrose only and has no action on the other carbohydrates that you mention.  The “sucrase” enzyme is an alpha-glucosidase which we sell as Alpha-Glucosidase (Maltase) E-MALTS.

Q16. Fructan Assay Procedure “Further treatment of extracts” Step 3, Pg 8 "if sample is cooled, it must be re-heated to 80?C to ensure fructans redissolve". If frozen to –20?C, thawed quickly & re-heated to 80?C, will the fructans re-dissolve completely?

Fructans are very soluble and will redissolve very readily.  They may precipitate on freezing (at least the polymeric portion) but they will easily re-dissolve at 80?C.

48瓶用的硅膠支持磁盤50張Shinn核心用|柴田科技有限公司-環境檢測設備、科學儀器的制造銷售

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康寧corning 3504 PLT,96W,WHT,PS,FILTER,W/0.2u P
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