磷酸轉乙酰酶[枯草桿菌] Phosphotransacetylase (Bacillus subtilis) 貨號:E-PTABS Megazyme中文站

磷酸轉乙酰酶[枯草桿菌]

英文名:Phosphotransacetylase (Bacillus subtilis)

貨號:E-PTABS

規格:3000 Units

High purity recombinant Phosphotransacetylase (B. subtilis) for use in research, biochemical enzyme assays and 
in vitro diagnostic analysis. 

EC 2.3.1.8

Acetyl-CoA: orthophosphate acteyltransferase; Phosphate acetyltransferase.

Recombinant from B. subtilis. 
In 3.2 M ammonium sulphate. This enzyme is commonly used, in conjunction with 
acetate kinase, for the measurement of acetic acid.

Specific activity: 18,325 U/mg (25oC, pH 7.4).

Stable at 4oC for > 2 years. 

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合成·反應裝置ケミスト廣場CPP – 2000系列用不銹鋼管1 / 8英寸2米|柴田科技有限公司-環境檢測設備、科學儀器的制造銷售

產品詳細

科學儀器

合成·反應裝置ケミスト廣場CPP – 2000系列用不銹鋼管1 / 8英寸2米

商品代碼其他情報(式樣)

這個產品比較表中追加
商品代碼 054310 – 2753
型式
價格(不含稅) 8,1000日元

上海金畔生物科技有限公司

686%2F%3Fc%3D34& ” 686%2F%3Fc%3D34&

16w/v%多聚甲醛溶液,無甲醇(Methanol free)25%戊二醛溶液


產品編號 產品名稱 產品規格 產品等級 產品價格
077-06271 25%?戊二醛溶液?25%?Glutaraldehyde?Solution 10ml×10A 電子顯微鏡用
071-02031 10%戊二醛溶液?10%?Glutaraldehyde?Solution 2ml×5A 電子顯微鏡用
072-02262 20%?戊二醛溶液?20%?Glutaraldehyde?Solution 25ml 電子顯微鏡用
076-02265 20%?戊二醛溶液?20%?Glutaraldehyde?Solution 500ml 電子顯微鏡用
161-20141 4%?多聚甲醛溶液,磷酸鹽緩沖溶液?4%?Paraformaldehyde?Phosphate?Buffer?Solution 100ml 組織固定用
163-20145 4%?多聚甲醛溶液,磷酸鹽緩沖溶液?4%?Paraformaldehyde?Phosphate?Buffer?Solution 500ml 組織固定用
015-25191 抗磷酸化α-突觸核蛋白,單克隆抗體(pSyn#64)?Anti?Phosphorylated?α-Synuclein, 50μl 免疫化學用
019-19741 抗Iba1,兔(免疫細胞化學用)?Anti?Iba1,?Rabbit?(for?Immunocytochemistry) 50μg 免疫化學用
014-24061 抗IDH1,單克隆抗體?Anti?IDH1,?Monoclonal?Antibody 100μg 免疫化學用
011-24071 抗IDH2,單克隆抗體?Anti?IDH2,?Monoclonal?Antibody 100μg 免疫化學用
018-24081 抗IDH1-R132H,單克隆抗體?Anti?IDH1-R132H,?Monoclonal?Antibody 100μg 免疫化學用
015-24091 抗IDH1-R132S,單克隆抗體?Anti?IDH1-R132S,?Monoclonal?Antibody 100μg 免疫化學用
018-24101 Anti-human?Podoplanin,單克隆抗體?Anti?HumanPodoplanin,?Monoclonal?Antibody 100μg 免疫化學用
015-24111 Anti?mousepodoplanin,單克隆抗體?Anti?mousePodoplanin,?Monoclonal?Antibody 100μg 免疫化學用
014-24821 抗CRMP2,單克隆抗體(9F)?Anti?CRMP2,?Monoclonal?Antibody?(9F) 100μl 免疫化學用

16w/v%多聚甲醛溶液,無甲醇(Methanol free)16w/v%多聚甲醛溶液,無甲醇(Methanol free)25%戊二醛溶液

25%戊二醛溶液

現出量少安瓿包裝(10支裝)

組織/細胞固定液

16w/v%多聚甲醛溶液,無甲醇(Methanol free)25%戊二醛溶液

  多聚甲醛具有較高的反應性和細胞通透性,被廣泛用作細胞中蛋白質和核酸的可逆交聯劑和細胞/組織固定劑。關于配制16w/v%多聚甲醛溶液,由于多聚甲醛難溶于水,生成甲醛需要一定時間和操作。

  本產品是已配制16w/v%多聚甲醛溶液,為1mL和10mL的安瓿包裝(惰性氣體密封),無需擔心儲存期間由于氧化產生甲酸,任何時候打開使用都是“剛制成”狀態。請配合25%戊二醛溶液一起使用。



◆優點

● 便利的一次性量小包裝!

  減少廢棄物的產生,降低對環境的影響。

● 加快實驗/研究的速度!!
  減少溶液配制的時間和操作,提高實驗效率。

● 適用于各種實驗應用!!!

  能應對組織/細胞的各種實驗。

 

◆應用實例

  用PBS等稀釋16w/v%多聚甲醛溶液,無甲醇后使用。


應用

使用濃度

稀釋

細胞/組織固定

4%

1:4

細胞/細胞器內交聯

1%

1:16

ChIP化驗

1%

1:16

流式細胞儀分析

2%

1:8

 

  可以使用Karnovsky,1965改良固定液(2%多聚甲醛、2.5%戊二醛)。


16w/v%多聚甲醛溶液

10mL

25%戊二醛溶液

8mL

0.2M 磷酸緩沖液(pH7.4)

40mL

蒸餾水

22mL

 

相關資料

16w/v%多聚甲醛溶液,無甲醇(Methanol free)25%戊二醛溶液

詳情請點擊圖片


相關產品

產品編號

名稱

規格

容量

071-02031

10%戊二醛溶液

10% Glutaraldehyde Solution

電子顯微鏡用

2ml×5A

072-02262

20% 戊二醛溶液

20% Glutaraldehyde Solution

電子顯微鏡用

25ml

076-02265

電子顯微鏡用

500ml

161-20141

4% 多聚甲醛溶液,磷酸鹽緩沖溶液

4% Paraformaldehyde Phosphate Buffer Solution

組織固定用

100ml

163-20145

組織固定用

500ml

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名稱

規格

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015-25191

抗磷酸化α-突觸核蛋白,單克隆抗體(pSyn#64)

Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64)

免疫化學用

50μl

019-19741

抗Iba1,兔(免疫細胞化學用)

Anti Iba1, Rabbit (for Immunocytochemistry)

免疫化學用

50μg

014-24061

抗IDH1,單克隆抗體

Anti IDH1, Monoclonal Antibody

免疫化學用

100μg

011-24071

抗IDH2,單克隆抗體

Anti IDH2, Monoclonal Antibody

免疫化學用

100μg

018-24081

抗IDH1-R132H,單克隆抗體

Anti IDH1-R132H, Monoclonal Antibody

免疫化學用

100μg

015-24091

抗IDH1-R132S,單克隆抗體

Anti IDH1-R132S, Monoclonal Antibody

免疫化學用

100μg

018-24101

Anti-human Podoplanin,單克隆抗體

Anti HumanPodoplanin, Monoclonal Antibody

免疫化學用

100μg

015-24111

Anti mousepodoplanin,單克隆抗體

Anti mousePodoplanin, Monoclonal Antibody

免疫化學用

100μg

014-24821

抗CRMP2,單克隆抗體(9F)

Anti CRMP2, Monoclonal Antibody (9F)

免疫化學用

100μl

011-24831

抗CRMP5,單克隆抗體(KZ19)

Anti CRMP5, Monoclonal Antibody (KZ19)

免疫化學用

100μl

010-24801

抗磷酸化CRMP1/2(絲氨酸522),兔

Anti Phosphorylated CRMP1/2 (Ser522), Rabbit

免疫化學用

100μl

 

麥芽Beta葡聚糖酶和纖維素內切酶檢測片劑 (200) Beta-Glucazyme (100mg) 1000 Tablets 貨號:T-BGZ-200T Megazyme中文站

麥芽Beta葡聚糖酶和纖維素內切酶檢測片劑 (200)

英文名:Beta-Glucazyme (100mg) 1000 Tablets

貨號:T-BGZ-200T

規格:200 Tablets

High purity dyed and crosslinked beta-Glucazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

RACI Standard Method. For the assay of malt ß-glucanase and endo-cellulase. Containing AZCL-Barley ß-glucan.

PDF Download

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日本關東化學Kanto 氯化鈣 CAS 10035-04-8

日本關東化學Kanto 氯化鈣 CAS 10035-04-8
日本關東化學代理商–上海金畔生物
代理優勢:貨期2-3周。
歡迎訪問關東化學株式會社Kanto官網或者咨詢我們獲取更多產品信息。
日本關東化學Kanto 氯化鈣 CAS 10035-04-8

Product No. 07058-00
Product name Calcium chloride dihydrate
Grade Guaranteed reagent for JIS
Purity 99.0%~103.0%(T)
Package 500g
Price ¥2,000
Stock 100<=
Product information ??JISK8122

日本關東化學Kanto 氯化鈣 CAS 10035-04-8

Product No. Product Name Grade Package Stock
07058-00 Calcium chloride dihydrate Guaranteed reagent for JIS 500g 100<=
07058-01 Calcium chloride dihydrate Extra pure 500g 100<=
07058-23 Calcium chloride dihydrate for molecular biology 100g 50<=
07058-80 Calcium chloride dihydrate Guaranteed reagent for JIS 15kg 50<=
07058-81 Calcium chloride dihydrate Extra pure 15kg 50<=
2382-1M Calcium chloride dihydrate EMSURER 250g non-stocked
58001-17 Calcium chloride 0‐2H2O Food additives 500g 100<=
58001-41 Calcium chloride 0‐2H2O Food additives 20kg Contact us
42352-1A Calcium chloride dihydrate   25g non-stocked
42352-2A Calcium chloride dihydrate   500g non-stocked

上海廠家直供2ML安瓶玻尿酸精華液保濕補水美白vc廣州藥妝OEM

上海廠家直銷安瓿瓶灌裝封口來料加工OEM代加工透明棕色適合精油精華原液

上海廠家直供2ML安瓶玻尿酸精華液保濕補水美白vc廣州藥妝OEM

安瓿瓶簡稱:安瓶(Ampoules)是無菌真空密封醫用包裝,非注射,達到隔絕外界任何細菌感染,適用于任何精華液,特別是無添加防腐劑精華液等對保鮮極高的化妝品。每一只安瓶玻尿酸精華液在產品主人玻尿酸精華液之后瓿口立即被加熱密封,每一只玻尿酸精華液都要經過多個階段的質量控制,以保證每一只玻尿酸精華液注入的是新鮮制成的活性精品。

名稱:玻尿酸精華液

規格:2ml

產品產地:德國

產品成分:0.35%玻尿酸、卵磷脂、維生素E、五肽-3、EGF、水、透明質酸鈉、水解膠原蛋白、牛油果數籽(AVOCADO)提取物、聚谷氨酸、谷胱甘肽。

產品 功能:補水、修復、抗氧化、收細毛孔、提亮、改善膚色暗啞、緊致。

適用膚質:任何膚質,使用時肌膚若有不適,請立即停止使用。

使用方法:潔面爽膚后使用,均勻涂抹于臉部及需要部位,輕拍至吸收即可。每天早上洗完臉一只,晚上洗完臉一只。3個星期一個療程

上海金畔生物科技有限公司
固話總機:021-50837765
訂貨熱線:15221999938

 













































上海金畔生物科技有限公司
固話總機:021-50837765
訂貨熱線:15221999938





五糖(X3Glc4) Heptasaccharide (X3 Glc 4) – 50mg 貨號:O-X3G4 Megazyme中文站

五糖(X3Glc4)

英文名:Heptasaccharide (X3 Glc 4) – 50mg

貨號:O-X3G4

規格:50 mg

CAS: 121591-98-8
Molecular Formula: C39H66O
33
Molecular Weight:
1062.9
Purity: > 90%

High purity Heptasaccharide X3Glc4 for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

 

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石油產品試驗試劑使用|一般分析純試劑(其他)|一般分析純試劑|試劑|關東化學株式會社

“揮發油等確保質量等関法律施行規則(品確法)”上,輕油中去的生物柴油燃料(Bio Diesel Fuel:BDF)的添加被承認。現在輕油,“BDF添加了輕油”和“BDF不添加輕油”存在著,其規格值和試驗方法的規定。我們這些考試您可以使用產品為您準備。

◎專用HPLC柱

產品名 包裝 產品編號
全能之硅60 BDF 250‐4.6(5μm)
Mightysil硅60 BDF 250‐4.6(5μm)
1 pk 25555 – 96

輕油中的脂肪酸甲酯/三酸甘油脂分離專用柱。“硬脂酸甲基”的保持時間:3.5分鐘以上,硬脂酸甲基和三里諾雷因的分離度:3以上的保證。

◎標準液

產品名 包裝 產品編號
硬脂酸甲酯/三里諾Rain混合標準原液
Methyl stearate / Trilinolein standard stock solution
5 mL×5 25192 – 96

硬脂酸甲基5克/ 50毫升+三里諾Rain 0.05 g / 50毫升(輕油溶液)

◎其他相關產品

產品名 包裝 產品編號
輕油注1)
Light oil
5 mL×5 24107 – 08
0.4 vol % 2 -丙醇/n‐己烷溶液注2)
0.4 vol % 2 – Propanol / n‐Hexane solution
一L 32459 : 79
3升 32459 – 76

注1)…脂肪酸甲酯及三酸甘油脂,不含柴油。標準溶液稀釋用使用。 注2)…脂肪酸甲酯及三酸甘油脂的分析方法中洗提液使用。

參考資料><

、「揮發油等確保質量等関法律施行規則一部改正省令問題”
經濟產業省資源能源廳(平成19年1月15日)
·脂肪酸???????及??????????分析方法 ………….. 液相色譜法
·脂肪酸???????混合軽油中的?????分析方法 ………….. 氧檢測式氣相色譜儀法
………….. 頂空―氣相色譜儀法
………….. 水抽出―氣相色譜儀法
·脂肪酸???????混合軽油中的甲酸,醋酸,???????酸分析方法 ………….. 水抽出-???????????法
·脂肪酸???????混合軽油 ………….. 氧化安定性的試驗方法

■追加項目系規格值及試驗方法

管理項目 BDF混合的輕油 BDF不輕油混合 分析試驗方法
現有的項目 硫分 0.005 w 1.5%以下 0.005 w 1.5%以下 2541 JIS K – 1,- 2,
– 6或- 7號
十六烷指數 45以上 45以上 JIS K 2280
90%蒸餾溫度 360度以下 360度以下 JIS K 2254
追加項目 脂肪酸甲酯含量 5.0 w 1.5%以下 0.1 w 1.5%以下 HPLC法 
甘油三酸酯含量 0.01 w 1.5%以下 0.01 w 1.5%以下
甲醇含量 0.01 w 1.5%以下 GC法
酸值 0.13 mgKOH / g以下 JIS K 2501
甲酸,醋酸,丙酸 計0.003 w 1.5%以下 IC法
氧化安定性(酸值增加部分) 0.12 mgKOH / g以下 氧化安定性的試驗方法
“揮發油等的品質確保関法律施行規則一部改正省令”比

■其他相關產品歐洲規格(EN)和美國材料試驗協會規格(ASTM)那么,FAME NEET,關于各自的形狀標準化,已在國內也去輕油混合基礎材料和啃老族FAME的性狀關于規格標準化被發展。 ◎ASTM D – 6584相關產品

產品名 包裝 產品編號
??????標準液
Glycerin standard solution
0.5毫克/ mLPyridine in 2毫升 *49804 – 64
??????標準液
Monoolein standard solution
5毫克/ mLPyridine in 2毫升 *49804 – 65
1,3 -??????標準液
1,3 – Diolein standard solution
5毫克/ mLPyridine in 2毫升 *49804 – 66
??????標準液
Triolein standard solution
5毫克/ mLPyridine in 2毫升 *49804 – 67
(<em>S</em> )-(-)- 1,2,4 -?????????標準液
(S )-(-)- 1,2,4 – Butanetriol standard solution
1毫克/ mLPyridine in 5毫升 *49804 – 68
???????標準液
Tricaprin standard solution
8毫克/ mLPyridine in 5毫升 *49804 – 69
N -???- <em>N</em> -(????????)?????????????
N – Methyl – N -( trimethylsilyl)trifluoroacetamide(MSTFA)
5克 22158 – 1 A

◎FAME相關產品

產品名 包裝 產品編號
???????????100(consumer grade)
Biodiesel 100
0.5毫克/ mLDichloromethane in 1毫升 *49804 – 70
20毫克/ mLDichloromethane in 1毫升 *49804 – 71
???????????20
Biodiesel 20
0.5毫克/ mLDichloromethane in 1毫升 *49804 – 72
20毫克/ mLDichloromethane in 1毫升 *49804 – 73
???????????100(refinery grade)
Biodiesel 100
0.5毫克/ mLDichloromethane in 1毫升 *49804 – 74
20毫克/ mLDichloromethane in 1毫升 *49804 – 75
AccuStandard社產品。進口產品。

■其他石油產品試驗用試劑◎石油制品及潤滑油-中和試驗法(JIS K 2501)

產品名 規格 包裝 產品編號
甲苯?水?2 -丙醇混液
Toluene·water·2 – propanol mixed solution
石油制品試驗用 500毫升 *41035 – 08
3升 41035 – 76
18 L *41035 – 84
醋酸·氯苯甲醛混液
Acetic acid·chlorobenzene mixed solution
石油制品試驗用 500毫升 25914 – 08
3升 25914 – 76
*訂貨產品。本公司營業所或銷售店為止請顯著。

◎揮發油中的氧化生成物的測量方法?揮發油中的氧化生成物的測量方法和經濟產業大臣規定的方法”,平成24年7月的告示而被修改,平成八年通商産業省告示第百十九號廢止了。被修改的測量方法使用“色譜儀調整液?和?揮發油極性成分配制溶液?的完畢的試劑產品化了。

產品名 規格 包裝 產品編號
調整液相色譜儀
Reference solution在gasoline oxidation products描述
石油制品試驗用 5 mL×5 36002 – 96
苯甲醇、苯甲酯、甲苯、二甲苯,アセトニトリル0.5:0.5:1.5:1.5:6.0的容量比
揮發油極性成分溶液
Gasoline polar components solution
石油制品試驗用 5 mL×5 17007 – 96
酚100 ppm(w / v )、m -甲酚100 ppm(v / v )、o -甲酚100 ppm(v v )、/2,3 -二甲苯酚100 ppm(w / v )、苯1000ppm(v / v)アセトニトリル溶液

◎香豆素分析純試劑輕油識別劑標準分析方法記載“香豆素”的分析,測量使用試劑類和“堿性”、“酒精液”發售。頻繁進行測量現場的利用的基礎上,各產品一起“L”3尺寸為您準備。

產品名 規格 包裝 產品編號
1 -丁醇·乙醇混合液
1 – Butanol·ethanol mixed solution
石油制品試驗用 3升 04460 – 76
酒精液… 1 -丁醇/乙醇= 4 / 3定量分析用
氫氧化鈉‐硝酸鈉溶液
Sodium hydroxide‐sodium nitrate solution
石油制品試驗用 3升 38185 – 76
堿性… 100毫升水溶液中NaOH:10克,NaNO 3 : 20克在內

◎石油制品及潤滑油-中和試驗法(JIS K 2501)相關的試劑JIS K 2501石油產品及潤滑油-中和值試驗方法被使用。

產品名 規格 包裝 產品編號
0.1 mol / L鹽酸(2 -丙醇性)( N / 10)
0.1 mol / L Hydrochloric acid,2 – propanolic(N / 10)
500毫升 *18597 – 08
甲基橙色溶液
Methyl orange solution
滴定法用指示劑 500毫升 *25860 – 08
100毫升 *25860 – 23
0.1 mol / L氫氧化鉀溶液(2 -丙醇性)( N / 10)
0.1 mol / L Potassium hydroxide solution,2 – propanolic(N / 10)
石油制品試驗用 500毫升 32871 – 08
p -萘酚本玉米蛋白溶液(2克)/ L醋酸溶液
p‐Naphtholbenzein solution
非水滴定法用 50毫升 28571 – 96
0.1 mol / L氯酸·醋酸溶液(N / 10)
0.1 mol / L Perchloric acid‐acetic acid solution(N / 10)
非水滴定法用 500毫升 32795 – 08
3升 32795 – 76
0.1 mol / L醋酸鈉-醋酸溶液
0.1 mol / L Sodium acetate‐acetic acid solution
非水滴定法用 500毫升 38004 – 08
*訂貨產品。本公司營業所或銷售店為止請顯著。

◎石油產品-燃料油-十六辛烷值試驗方法(JIS K 2280)JIS K 2280石油產品-燃料油-辛烷及十六辛烷值試驗方法以及十六烷指數計算方法,標準作為燃料被使用的正庚烷,2,2,4 -三甲基戊二(異辛烷)為『辛烷值測定用』而準備著。

產品名 規格 包裝 產品編號
n六癸烷十六烷
nHexadecane‐
十六辛烷值測量 500毫升 18563 – 08
庚烷(<em>n -</em>庚烷)
Heptane(n‐Heptane)
辛烷值測量 12公斤 18005 – 82
2,2,4 -三甲基戊烷
2,2,4 – Trimethylpentane
辛烷值測量 12公斤 31005 – 82

◎航空潤滑油試驗方法(JIS K 2503)相關的試劑

產品名 規格 包裝 產品編號
0.5 mol / L鹽酸(N / 2)
0.5 mol / L Hydrochloric acid(N / 2)
500毫升 18593 – 08
3升 18593 – 76
0.1 mol / L鹽酸(N / 10)
0.1 mol / L Hydrochloric acid(N / 10)
500毫升 18595 – 08
容量分析用 一L 18595 : 79
3升 18595 – 76

◎原油及石油制品硫分試驗用(JIS K 2511)相關的試劑

產品名 規格 包裝 產品編號
0.05 mol / L氫氧化鈉溶液(N)/ 20
0.05 mol / L Sodium hydroxide solution(N)/ 20
500毫升 37852 – 08
硫酸鉀
Potassium sulfate
特級 500克 32403 : 00
特級 100克 32403 – 20
特級 25克 32403 – 30

◎FIA分析純試劑

產品名 規格 包裝 產品編號
熒光指示劑添加凝膠
Fluorescent indicator dyed gel
石油制品試驗用 40克 * *49400 – 14
* *進口產品。庫存信息,本公司營業所或銷售店為止請顯著。

石油產品試驗試劑使用手冊(228 KB)

試劑的咨詢

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膳食纖維總量檢測試劑盒 Total Dietary Fibre Assay Kit 貨號:K-TDFR-200A Megazyme中文站

膳食纖維總量檢測試劑盒

英文名:Total Dietary Fibre Assay Kit

貨號:K-TDFR-200A

規格:200 assays per kit

分析物意義:小腸不能消化的碳水化合物

Megazyme檢測試劑盒優點:試劑穩定。成本低。AOAC方法985.29和991.43;AACC方法32-07和32-21

The Total Dietary Fiber test kit is suitable is suitable for the measurement and analysis of Total Dietary Fiber.

For the determination of Total Dietary Fiber in cereal products,
foodstuffs, feeds and other materials

Principle:
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose

(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following alcohol
precipitation

(4) Ash and residual protein determined on DF residues
and subtracted

Kit size: 100 / 200 assays
Method: Hydrolysis / removal of non-dietary
fibre components
Total assay time: ~ 100 min
Detection limit: 0.5-100% of sample weight
Application examples:
Food ingredients, food products and other materials
Method recognition:
AOAC (Methods 985.29, 991.42, 991.43 and 993.19), AACC
(Methods 32-05.01, 32-06.01, 32-07.01 and 32-21.01) and
CODEX (Type I Method)

Total Dietary Fiber Assay Kit, for the measurement and analysis of total, soluble and insoluble dietary fiber according to AOAC and AACC approved methods. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.

Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.

2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.

3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

Two separate methods are described in the associated data booklet:

METHOD 1:

DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER

Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

METHOD 2:

DETERMINATION OF TOTAL DIETARY FIBER

Based on AACC method 32-05.01 and AOAC Method 985.29.

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years
  • High purity / standardised enzymes employed
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. What is the stability of enzymes for Total Dietary Fibre method?

The enzymes are very stable.  In fact on storage at room temperature for 10 months, the decrease in  activity of thermostable alpha-amylase and amyloglucosidase is less than 10% and the decrease in activity in protease is about 40%.  When stored at 4?C, the enzymes are stable for about 5 years, and at -20?C they are stable for more than 10 years.

Q3. I was wondering if Megazyme has a kit to determine insoluble and soluble dietary fibre in barley?

Our kit is applicable to the measurement of Soluble, Insoluble and Total Dietary Fibre as per the AOAC/AACC methods.  The main value of our kit is that the Amyloglucosidase is free of Cellulase, i.e. the Beta-Glucan will not be degraded and underestimated.

Q4. Are the lower temperatures of 90?C to 95?C adequate for the hydrolysis if we extend the incubation time?

The main reason for the stated 100?C is to ensure that the starch is gelatinised and hydrolysed by alpha-amylase.  We are sure that a temperature of 95?C should be fine.

Q5. Is it necessary to do simultaneously nitrogen and ash analysis or can the test be applied to samples already analysed for these two characters?

It is necessary to measure the nitrogen in the residue.  Most protein is removed by protease action.  We think it best to follow the AOAC method as closely as possible.

Q6. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q7. Is there a preferred rate of heating and cooling for the recommended crucible (Corning® No. 32940-50C or equivalent) to avoid breakages?

The crucible has the potential to break if heated or cooled too rapidly . The graph below shows the manufacturer’s recommended heating and cooling profile for safe use with the crucible.膳食纖維總量檢測試劑盒 Total Dietary Fibre Assay Kit 貨號:K-TDFR-200A  Megazyme中文站
 

Q8. How should I prepare the required 78% ethanol solution?

A volume reduction occurs on mixing water with 95% ethanol (or IMS). 1 L of 78% ethanol is prepared by adding 821 mL 95% ethanol (or IMS) to 207 mL H2O.
If using a 1 L volumetric flask, this is best accomplished by placing 821 mL 95% ethanol (or IMS) into the volumetric flask. Dilute to volume with deionised water. Mix well. Check the level and if necessary add more deionised water to bring it back up to the 1 L mark.