Sigma 孕酮Progesterone

Sigma 孕酮Progesterone

產品編號 P8783 | CAS號 57-83-0 | SIGMA

英文名:Progesterone
中文名:1.孕甾酮 2.黃體酮 3.黃體素?4.孕酮? 5.孕醇 6.黃體激素
Synonym: 4-Pregnene-3,20-dione
CAS Number 57-83-0
Empirical Formula (Hill Notation) C21H30O2
Molecular Weight 314.46
description:synthesized from materials derived from plant source
product line:BioReagent
form:powder
potency:0.1-20 ng/mL
mp:128-132 °C(lit.)
solubility :absolute ethanol: soluble1 mg/mL
suitability :uitable for cell culture

Application

用于細胞培養,研究細胞信號傳導和基因表達的甾醇調控

Other Notes

由卵巢黃體產生的類固醇激素。

包裝:1, 5, 25 g in glass bottle

Reconstitution

To prepare 20μg/ml stock solution, add 1ml absolute ethanol per mg progesterone, gently swirl to dissolve, add 49ml sterile medium per ml ethanol.

Physical form

室溫下為粉末狀;分成小份凍存,以避免使用時反復冷凍/解凍

Biochem/physiol Actions

誘導子宮內皮細胞的成熟和分泌活動;抑制排卵。孕酮與乳腺癌的病因有關。

Sigma人血管緊張素 II

Sigma人血管緊張素 II

產品編號 A9525 | CAS號 4474-91-3 | SIGMA

Angiotensin II human ? ?≥93% (HPLC), powder

CAS號 4474-91-3
分子式: C50H71N13O12
分子量 1046.18
assay??? ≥93% (HPLC)
form??? powder
total impurities??? 1.2-10.4% acetate
storage temp.? -20°C
Amino Acid Sequence
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
Antibody Adsorbant
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-OH · 2CH3COOH · H2O
Analysis Note
肽含量不低于 75%
Biochem/physiol Actions
血管緊張素 (Ang) II 在調節心血管的血液動力學和結構中起重要作用。Ang II 在成人組織內的大部分已知效應均可歸因于血管緊張素 II 類型 I (AT1) 受體。AT1 和 AT2 受體在藥理學和生物化學性質上有差別,在心血管血液動力學和細胞生長方面表現出相反的效應。此外,腎素-血管緊張素和一氧化氮生成系統在心血管功能的調節中顯示出相互作用的現象。Ang II 刺激血管生成,增加微血管密度。事實上,血管緊張素 II 是由血管緊張素轉換酶對血管緊張素原的作用而生成;C-端-組氨酰-亮氨酸裂解。
保存條件: -20°C

Sigma絲裂霉素 C 來源于頭狀鏈霉菌

Sigma絲裂霉素 C 來源于頭狀鏈霉菌

產品編號 M0503-2mg?| CAS號 50-07-7 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

Biochem/physiol Actions

DNA 合成、細胞核分裂和癌細胞的抑制劑。對革蘭氏陽性菌、革蘭氏陰性菌、抗酸桿菌有抗菌性。

Caution

This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared – the precipitated solution has been proven toxic to cells.

Preparation Note

Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 阿霉素 鹽酸鹽

Sigma 阿霉素 鹽酸鹽

產品編號 D1515 | CAS號 25316-40-9 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

包裝:10 mg in glass bottle?Biochem/physiol Actions

Naturally fluorescent anthracycline antibiotic, anticancer drug. Doxorubicin is a substrate of MRP1 which was first cloned from a DOX-resistant lung cancer cell line. Fluorescent property has been exploited for the measurement of drug efflux pump activities as well as resolving the important question of intracellular localization of various multidrug resistance proteins and the role of subcellular organelles (Golgi and lysosome) in the sequestration of drugs and its implication in drug resistant phenotypes.

法律信息

Adriamycin 為以下機構的注冊商標: RDF Pharmacia & Upjohn S.P.A.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antibiotics by Application, Antineoplastic and Immunosuppressive Antibiotics,

更多…
assay 98.0-102.0% (HPLC)
mp 216?°C (dec.)(lit.)
solubility H2O: soluble50?mg/mL, clear
Mode of action DNA synthesis | interferes
antibiotic activity spectrum viruses
storage temp. 2-8°C

Sigma 放線菌素 D

Sigma 放線菌素 D

產品編號 A9415 | CAS號 50-76-0 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.

Caution

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

包裝:2, 5, 10, 25 mg in glass bottle

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 二甲基亞砜DMSO

Sigma 二甲基亞砜DMSO

產品編號 D2650 | CAS號 67-68-5 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

DMSO 是一種用于化學反應(如聚合酶鏈式反應 (PCR))的極性非質子溶劑,也可作為冷凍保護玻璃化劑用于保存細胞、組織和器官。DMSO 可在細胞冷凍培養基中用于保護細胞免受冰晶導致的機械損傷。它可用于原代、繼代培養和重組的異倍體和雜交瘤細胞系、胚胎干細胞 (ESC) 以及造血干細胞的凍存。DMSO 常與 BSA 或胎牛血清 (FBS) 一同使用。

Caution

容易過度冷卻,并在室溫下緩慢融化固化的產品放在室溫下可以再次液化,對其本身無影響。

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

包裝:5mL 和 10mL 火焰封口安瓿瓶包裝,100mL 棕色瓶包裝

sigma 醋氨酚BioXtra

sigma 醋氨酚BioXtra

產品編號 A7085 | CAS號 103-90-2 | SIGMA-ALDRICH

Application:鎮痛劑。

包裝:1 kg in glass bottle,100, 500 g in glass bottle

Related Categories Building Blocks, C6 to C8, Chemical Synthesis, Organic Building Blocks, Oxygen Compounds,

更多…
product line BioXtra
assay ≥99.0%
form powder
impurities ≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter
ign. residue ≤0.1%
mp 168-172?°C(lit.)

sigma-白蛋白 來源于牛血清

sigma-白蛋白 來源于牛血清

產品編號 A3803 | CAS號 9048-46-8 | SIGMA

包裝:10, 50, 100 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

采用熱休克分級分離法制備。經過進一步純化以降低脂肪酸含量。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Related Categories Albumin, Bovine Serum Albumins, Heat Shock Fractionated, 生化試劑, 蛋白質及衍生物 更多…
assay ≥98% (agarose gel electrophoresis)
form lyophilized powder
mol wt mol wt ~66?kDa
purified by heat shock fractionation
packaging poly bottle of
origin USA origin
impurities ≤0.005% Fatty acids

 

產品編號 A8806 | CAS號 9048-46-8 | SIGMA

包裝:1, 5 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

通常稱為 Cohn fraction V;本品采用改進的 Cohn 氏冷乙醇分級分離法制備。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.[]

Protocols & Applications

Select the right albumin for your application with the Albumin Selection Guide

Related Categories Albumin, Albumins, Albumins and Transport Proteins,Albumins by Application, Bioactives and Supplements for Stem Cell Expansion,

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Quality Level PREMIUM
product line BioReagent
assay ≥96% (agarose gel electrophoresis)
form lyophilized powder
mol wt mol wt ~66?kDa
purified by cold ethanol fractionation
packaging poly bottle of

Sigma特殊產品Thiopropyl–Sepharose? 6B

Sigma特殊產品Thiopropyl–Sepharose? 6B

產品編號 T8387 | CAS號 68517-67-9 | SIGMA

包裝:1, 5, 15 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose and dextran

Application

Thiopropyl Sepharose? 6B is used in protein chromatography, affinity chromatography and hydrophobic interactions. Thiopropyl Sepharose? 6B has been used for the removal of inhibitors from PCR extracts from evidence collected for law enforcement murder investigations. Thiopropyl Sepharose? 6B has also been used to describe a method for the investigation of functional properties of distinct domains of viral thiol proteins, including the influenza virus membrane M1 protein.

法律信息

Sepharose is a registered trademark of GE Healthcare