Wako 031-17601 膠原酶 I型 Collagenase Type I

Wako 031-17601 膠原酶 I型 Collagenase Type I

【產品名稱】Collagenase Type I

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥

【制造商】Wako Pure Chemical Industries, Ltd.

【銷售商代碼】031-17601

【規格】100mg

【CAS.NO】9001-12-1

【保存條件】Keep at RT.

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

【外觀】Brown, crystalline powder – powder

【注意事項】This product is for research use only. Do not administer it to human.

Source: Clostridium histolyticum

Solubility: The 1 mg is dissolved in 1 mL of water.

This is an enzyme that specifically hydrolyzes collagen as a substrate

and the activities of collagenase, caseinase, clostripain and trypsin are contained in good balance. It is mainly used for isolation of cells from epithelial tissue, lung and adipose tissue.

ES·iPS Catalog 2013

產品描述

膠原酶(Collagenase)是一種蛋白酶,一種肽鏈內切酶,能夠特異性的識別Pro-X-Gly-Pro序列(該序列高頻率出現在膠原中,很少發現于其他蛋白中)并切割該序列中性氨基酸(X)和甘氨酸(Gly)之間的肽鍵。許多蛋白酶都能水解單鏈且變性的膠原多肽,但膠原酶是唯一一種可以降解具有三股超螺旋結構的天然膠原纖維的蛋白酶,這種膠原纖維廣泛存在結締組織內。

本品是一種酶粗提物,不僅含有膠原酶(更準確的稱法為梭菌蛋白酶A(clostridiopeptidase A)),能夠降解天然膠原和網狀纖維。還含有其他的一些蛋白酶、多糖酶、脂酶等,分別能夠有效水解結締組織和上皮組織細胞外基質內的其他蛋白,多糖和脂質,使得本品非常適用于組織消化。

目前商業化提供的細菌膠原酶主要根據膠原酶活性的差異,分為四種類型:膠原酶I型,II型,III型和IV型,在應用上有所偏向:1)膠原酶I(Type I Collagenase):含有比較均勻的各種酶活力(包括膠原酶、酪蛋白酶、梭菌蛋白酶、胰蛋白酶活性)。通常用作上皮細胞、肝、肺、脂肪和腎上腺組織細胞的制備;2)膠原酶II(Type II Collagenase):含有更高的梭菌蛋白酶活性,通常用于心臟、骨、肌肉、胸腺和軟骨等組織來源細胞的制備;3)膠原酶III(Type III Collagenase):含有較低的蛋白酶活性,常用于乳腺細胞的制備;4)膠原酶IV(Type IV Collagenase):含有低胰酶活性,通常用于胰島細胞的制備,或者需要維持受體完整性的細胞制備實驗。

本品為I型膠原酶,可用于上皮、肝、肺、脂肪和腎上腺等組織和細胞的解離。

酶活力單位定義

在37℃,pH7.5 的條件下,5h內水解膠原產生相當于1μM L-亮氨酸的酶量定義為1個酶活力單位。

運輸和保存方法

室溫運輸。4℃避光保存,2年有效。儲存液-20℃避光凍存。

注意事項

為了您的安全和健康,請穿實驗服并戴一次性手套操作。

使用方法

  1. 膠原酶儲存液的配制

向每管100mg的膠原酶中加入100μL的含Ca2+、Mg2+的HBSS(Hank’s平衡鹽溶液,含Ca2+、Mg2+),輕輕旋渦震蕩使其充分溶解,制備成1g/ml(即1000×)的儲存液。然后用低蛋白結合性的0.22μm的濾膜過濾除菌,分裝成小份量,然后于-20℃避光凍存。

使用前于冰上解凍,避免反復凍融。其用于組織和細胞分散的常用濃度為:0.5-2.5mg/ml,用于軟骨消化的常用濃度為1-2mg/ml,需要根據特定的實驗條件或者參考相應的文獻資料確定所需的最佳工作濃度。

2.組織的分離

1)使用無菌手術刀或剪刀將組織切成3-4mm大小的組織塊;

2)利用含Ca2+、Mg2+的HBSS洗滌組織塊數次;

3)加入足量的含Ca2+、Mg2+的HBSS,使其浸沒組織塊,并加入膠原酶至需要工作濃度;

4)于37℃孵育4-18h。消化時使用水平搖床以及用3mM的CaCl2補充消化可以提高消化效率。

5)?已分散開的細胞可使用不銹鋼或尼龍網篩篩得,收集備用。未完全解離的組織另外添加適量的新鮮膠原酶工作液于37℃繼續孵育;

6)利用不含膠原酶的HBSS洗滌收集的細胞數次;

7)細胞培養液重懸上述細胞,利用自動細胞計數器或其他方法計算活細胞密度。

8)于細胞培養皿上利用合適細胞培養基接種細胞。

3.?器官灌注

1)向37℃預熱的含Ca2+、Mg2+的HBSS中加入膠原酶,另添加3mM的CaCl2有助于提高分離效率;

2)按照已優化的速率對相應的器官灌注膠原酶工作液;

3)將上述過程中回收的灌注液流經不銹鋼或尼龍網篩,從而將已解離的細胞或小片段組織塊與較大團塊分離開來,未充分解離的組織需利用新鮮膠原酶工作液于37℃進一步孵育;

4)利用不含膠原酶的HBSS洗滌收集的細胞數次;

5)細胞培養液重懸上述細胞,利用自動細胞計數器或其他方法計算活細胞密度。

6)于細胞培養皿上利用合適細胞培養基接種細胞。

 

 

貨號???品名?????規格? Cas

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

012-01965?? Alminium Oxide?? 500g???? –

012-03305?? Ammonium Dihydrogenphosphate? 500g???? 7722-76-1

012-08643?? Amino Acids Mixture Standard Solution, Type B 1mL×5A –

013-08391?? Amino Acids Mixture Standard Solution, Type H 5mL????? –

015-14461?? Amino Acids Mixture Standard Solution, Type AN-2? 5mL????? –

015-25191?? Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64)????? 50ul????? –

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

011-14463?? Amino Acids Mixture Standard Solution, Type AN-2? 1mL×5A –

011-19365?? 2,2′-Azobis[2-(2-imidazolin-2-yl)propane]

Dihydrochloride?? 500g???? 27776-21-2

016-08641?? Amino Acids Mixture Standard Solution, Type B 5mL????? –

016-20001?? Anti Iba1, Rabbit (for Western Blotting) ??? 50μg???? –

016-23281?? Anti Rat P2X4, Monoclonal Antibody???? 50ug???? –

017-19362?? 2,2′-Azobis[2-(2-imidazolin-2-yl)propane] Dihydrochloride ?? 25g 27776-21-2

018-08721?? Agar, Powder???? 100g???? 9002-18-0

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

018-22021?? Anti Mouse Ago2, Monoclonal Antibody 100μL??? –

019-03435?? Ammonium sulfate??? 500g???? 7783-20-2

019-19741?? Anti Iba1, Rabbit (for Immunocytochemistry)??? 50μg???? –

019-22291?? Anti Olfactory Marker Protein, Goat????? 100μL??? –

021-02195?? Boric acid???? 500g???? 10043-35-3

021-16201?? BES-H2O2(Cell-impermeant)?? 1mg????? –

031-17601?? Collagenase Type I??? 100mg?? 9001-12-1

032-08385?? Citric Acid Monohydrate?? 500g???? 5949-29-1

032-15372?? Casein Phosphopeptide??? 25g –

034-06363?? N-Caprylic Acid?? 25mL??? 124-07-2

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

034-16111?? Cefotaxime Sodium Salt?? 1g?? 64485-93-4

035-04595?? Creatinine??? 500g???? 60-27-5

035-17604?? Collagenase Type I??? 1g?? 9001-12-1

038-23221?? α-Casein, from Bovine Milk, Dephosphorylated? 1mg????? –

039-01335?? Carboxymethyl Cellulose Sodium Salt ? 500g???? 9004-32-4

021-17801?? BES-So-AM 1mg????? 936356-51-3

022-07985?? γ-Butyrolactone? 500mL?? 96-48-0

025-03195?? Buffer Solution Standard (Phosphate pH Standard Equimolal Solution)

pH6.86 (25 degrees C)??? 500mL?? –

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

047-18863?? Dexamethasone 100mg?? 50-02-2

049-18801?? 4′,6-Diamidino-2-phenylindole Dihydrochloride n-Hydrate????? 10mg????? 28718-90-3

058-07681?? EasySeparator??? 1set????? –

025-03195?? Buffer Solution Standard (Phosphate pH Standard Equimolal Solution)

pH6.86 (25 degrees C)??? 500mL?? –

019-08393?? Amino Acids Mixture Standard Solution, Type H 5Ax1mL –

019-11941?? Active Carbon-impregnated Silica Gel ? 10g –

019-19741?? Anti Iba1, Rabbit (for Immunocytochemistry)??? 50μg???? –

025-15481?? BES-Thio???? 1mg????? –

026-16371?? BF-170? 1mg????? –

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

027-01276?? Benzyl Alcohol??? 500mL?? 100-51-6

028-03185?? Buffer Solution Standard (Phthalate pH Standard Solution)

pH4.01 (25 degrees C)??? 500mL?? –

028-03185?? Buffer Solution Standard (Phthalate pH Standard Solution)

pH4.01 (25 degrees C)??? 500mL?? –

028-03205?? Buffer Solution Standard (Tetraborate pH Standard Solution)

pH9.18 (25 degrees C)??? 500mL?? –

077-03155?? Gelatin?? 500g???? 9000-70-8

081-00136?? Heparin Sodium? 10mu??? 9041-08-1

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

088-00705?? L-Histidine Hydrochloride Monohydrate ???? 500g???? 5934-29-2

093-06471?? Insulin, Human, recombinant?? 50mg??? –

097-06474?? Insulin, Human, recombinant?? 1g?? 11061-68-0

120-04891?? L-012??? 100mg?? –

125-05061?? Lysyl Endopeptidase, Mass Spectrometry Grade ???? 5×20μg? –

125-05061?? Lysyl Endopeptidase, Mass Spectrometry Grade ???? 5×20μg? –

125-05061?? Lysyl Endopeptidase, Mass Spectrometry Grade ???? 5×20μg? –

129-02541?? Lysyl Endopeptidase( R)?? 10AU???? –

129-02541?? Lysyl Endopeptidase( R)?? 10AU???? –

129-02541?? Lysyl Endopeptidase( R)?? 10AU???? –

129-04861?? LY 294002?? 5mg????? 154447-36-6

130-07871?? Melibiose???? 5g?? 585-99-9

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

028-16211?? BES-So (Cell-impermeant)????? 1mg????? –

028-17811?? BES-H2O2-Ac???? 1mg????? –

029-16361?? BF-168? 1mg????? –

039-23511?? CultureSure? Freezing Medium??? 100ml??? –

041-18861?? Dexamethasine?? 1g?? 50-02-2

042-29445?? Dibasic Sodium Phosphate Hydrate 500g???? 10039-32-4

043-22611?? Dextran 200,000 100g???? 9004-54-0

064-05381?? Fibroblast Growth Factor (basic)(FGFb / bFGF / FGF2),

Human, recombinant, Animal-derived-free? 50ug???? 106096-93-9

071-02293?? gamma-Globulin, from Human Serum?? 5g?? 9007-83-4

075-03075?? Gellan Gum? 500g???? 71010-52-1

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

131-00405?? Magnesium Sulfate??? 500g???? 10034-, 99-8

136-03032?? Methyl Red?? 25g 493-52-7

137-06085?? Molecular Sieves 4A1/16? 500g???? 70955-01-0

138-07872?? Melibiose???? 25g 585-99-9

145-08221?? Nuclease P1 500u???? 54576-84-0

145-08221?? Nuclease P1 500u???? 54576-84-0

162-09115?? Polyethylene Glycol 4,000 500g???? 25322-68-3

163-03545?? Potassium Chloride??? 500g???? 7447-40-7

164-21655?? N/400 Potassium Polyvinyl Sulfate Solu 500mL?? 26837-42-3

191-01785?? Trisodium Citrate Dihydrate??? 500g???? 6132-04-3

193-02041?? Glycerol 2-Phosphate Disodium Salt n-Hydrate?? 100g???? 819-83-0

195-12854?? Streptavidin, from Streptomyces avidinii???? 5mg????? 9013-20-1

195-16031?? StemSure (R) Freezing Medium???? 100ml??? –

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

196-00015?? Sucrose 500g???? 57-50-1

196-08675?? Sodium Dodecyl Sulfate??? 500g???? 151-21-3

197-07485?? Sodium Sulfate?? 500g???? 7757-82-6

199-02825?? Sodium Dihydrogenphosphate Dihydrate ?? 500g???? 13472-35-0

203-17561?? Trichostatin A???? 1mg????? 58880-19-6

207-00055?? L(+)-Tartaric Acid???? 500g???? 87-69-4

208-08385?? 1mol/l Triethylammonium Hydrogencarbonate Solution?? 500mL?? 15715-58-9

223-02112?? VA-044? 25g 27776-21-2

225-02111?? VA-044? 100g???? 27776-21-2

165-09105?? Polyethylene Glycol 2,000 500g???? 25322-68-3

165-17035?? Polyvinylpyrrolidone K 30 500g???? 9003-39-8

167-09045?? Polyethylene Glycol 200?? 500g???? 25322-68-3

169-04245?? Potassium Dihydrogen Phosphate? 500g???? 7778-77-0

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

169-09125?? Polyethylene Glycol 6,000 500g???? 25322-68-3

185-01003?? Rifampicin??? 5g?? 13292-46-1

186-01114?? all-trans-Retinoic Acid 50mg??? 302-79-4

189-01001?? Rifampicin??? 1g?? 13292-46-1

190-05535?? Trisodium Citrate Dihydrate??? 500g???? 6132-04-3

191-01665?? Sodium Chloride? 500g???? 7647-14-5

227-01071?? Valproic Acid 5g?? 99-66-1

231-02011?? Wetting Tension Test Mixture No.52.0? 50mL??? –

232-01941?? Wetting Tension Test Mixture No.42.0? 50mL??? –

233-01971?? Wetting Tension Test Mixture No.45.0? 50mL??? –

234-02001?? Wetting Tension Test Mixture No.50.0? 50mL??? –

235-01931?? Wetting Tension Test Mixture No.41.0? 50mL??? –

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

235-02031?? Wetting Tension Test Mixture No.56.0? 50mL??? –

236-01961?? Wetting Tension Test Mixture No.44.0? 50mL??? –

238-01921?? Wetting Tension Test Mixture No.40.0? 50mL??? –

238-02021?? Wetting Tension Test Mixture No.54.0? 50mL??? –

238-02141?? Wetting Tension Test Mixture No.73.0? 50mL??? 7732-18-5

253-00513?? Y-27632 5mg????? 331752-47-7

257-00511?? Y-27632 1mg????? 331752-47-7

290-34251?? Presep-C DNPH?? 20EA???? –

296-63801?? LabAssay? Phospholipid?? 1300tests???? –

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

298-64101?? Fructooligosaccharides standard set???? set? –

302-93561?? Phos-tag (TM) Agarose??? 0.5mL??? –

304-93521?? Phos-tag (TM) Acrylamide AAL-107????? 10mg??? –

304-93521?? Phos-tag (TM) Acrylamide AAL-107????? 10mg??? –

290-34251?? Presep-C DNPH?? 20EA???? –

290-63701?? LabAssay? Triglyceride??? 1000tests???? -2016-2-29

290-65901?? LabAssay? Creatinine????? 500tests –

291-58601?? Lab Assay ALP??? 900tests –

293-51601?? Endotoxin Extracting Solution for LAL Test? 10mL x 4???? –

294-62001?? DsDD cDNA Subtraction kit Wako?? 5tests??? –

294-63601?? LabAssay? NEFA 750tests –

294-65801?? LabAssay? Cholesterol ?? 1000tests???? –

295-50201?? DNA Extractor? Kit?? 50tests? –

295-51301?? Limulus ES-II Single Test Wako???? 25 tests –

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

295-51301?? Limulus ES-II Single Test Wako???? 25 tests –

295-73401?? Fructooligosaccharides Set????? Set? –

304-93526?? Phos-tag (TM) Acrylamide AAL-107 5mM Aqueous Solution?? 0.3ml??? –

305-93551?? Phos-tag? Mass Analytical Kit set? –

308-93541?? Phos-tag (TM) Biotin BTL-105 10mg??? –

308-93563?? Phos-tag (TM) Agarose??? 3mL????? –

308-97201?? Phos-tag Biotin BTL-1111 1mM Aqueous Solution??? 0.1000mL??? –

325-88521?? Lipase PS Amano SD? 10g –

298-65701?? LabAssay? Glucose?? 1000tests???? –

300-93523?? Phos-tag (TM) Acrylamide AAL-107????? 2mg????? –

301-93531?? Phos-tag (TM) Biotin BTL-104 10mg??? –

301-99413?? Phos-tagTM Biotin BTL-104 1mM Aqueous Solution[for Campaign]???? 0.1mL????? 753451-66-0

302-93522?? Phos-tagTM Acrylamide AAL-107 5mM Aqueous Solution[for Campaign]????? 0.3mL??? 871839-54-2

631-00651?? Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)???? 20mL??? –

Wako??031-17601??Collagenase Type I 9001-12-1???和光純藥?現貨

637-00653?? Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)???? 100mL?? –

637-00653?? Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)???? 100mL?? –

638-01021?? Endotoxin Standad /JPSE? 10000un????? –

986-10001?? Anti asialo GM1 (Rabbit), (014-09801)? 1mL ???? 617-45-8