Sigma絲裂霉素 C 來源于頭狀鏈霉菌

Sigma絲裂霉素 C 來源于頭狀鏈霉菌

產品編號 M0503-2mg?| CAS號 50-07-7 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

Biochem/physiol Actions

DNA 合成、細胞核分裂和癌細胞的抑制劑。對革蘭氏陽性菌、革蘭氏陰性菌、抗酸桿菌有抗菌性。

Caution

This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared – the precipitated solution has been proven toxic to cells.

Preparation Note

Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information